GK103 ScienCell 人前脂肪细胞生物学qPCR检测试剂盒，Human Preadipocyte Cell Biology qPCR Array Kit

ScienCell 研究实验室（ScienCell）是一家生物技术公司，其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞，细胞培养基和试剂，培养基添加因子，细胞来源的RNA，cDNA和蛋白质。ScienCell的科学家们以改善生活质量，实现细胞的能力，打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥，公司成立于1999年。

北京作为ScienCell品牌的特约代理商，为客户提供，正规进口，保证原装正品，货期稳定，折扣好的服务标准。

GK103 ScienCell人前脂肪细胞生物学qPCR检测试剂盒，Human Preadipocyte Cell Biology qPCR Array Kit，产品简介：

产品品牌： ScienCell

产品货号：GK103

产品名称：GK103 ScienCell人前脂肪细胞生物学qPCR检测试剂盒，Human Preadipocyte Cell Biology qPCR Array Kit

产品规格：One 96-well plate, one 20ul reaction per well

北京裕恒丰科技有限公司是ScienCell中国区总代理，提供各种基因表达分析相关品，ScienCell细胞培养基、转染试剂、细胞检测试剂盒等细胞生物学产品。

GK103 ScienCell人前脂肪细胞生物学qPCR检测试剂盒，Human Preadipocyte Cell Biology qPCR Array Kit， 产品说明：

前脂肪细胞是成纤维细胞样前体细胞，可以增殖并分化成成熟的脂肪细胞。前脂肪细胞在代谢稳态和营养信号传导中起重要作用。ScienCell 的 GeneQuery™ 人类前脂肪细胞生物学 qPCR 阵列试剂盒 （GQH-PAD） 旨在促进参与人类前脂肪细胞生物学的 88 个关键基因的基因表达谱分析，例如细胞增殖、分化、ECM 形成和脂质代谢。与肥胖和脂肪肉瘤有关的基因也包含在阵列中。下面显示了如何根据其功能对基因进行分组的简要示例：

前脂肪细胞标志物：ADIPOQ，LEP，HOXC8 / 9，TBX1，TMEM26，UCP1，CIDEA，PRDM16，SLC7A10，P2RX5

增殖： CCND1， PROKR1， LYRM1， FTO， SLC2A4， PDGFA， IL4， IGF1， FGF5， MAPK1/3， TNFSF10

分化和脂肪生成：PPARG，CEBPA/B，KLF2/5/15，SOCS1/3，INSR，DLK1，ADD1，SREBF1

ECM 和细胞骨架： MMP1/2/3， FAP， DPP4， TGFB1， COL1A1/2， SMAD2/4

脂质代谢： FABP5/7， LBP， LPL， PLIN2， ACSL1， LIPC

肥胖症： MC4R， UCP3， ADRB2/3， POMC， FFAR4， CARTPT

脂肪肉瘤： FUS， MDM2， HMGA2， DDIT3， CDK4， EWSR1， DES， VIM

注意：所有基因名称都遵循人类基因组组织基因命名委员会（HGNC）的符号。

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪，每个孔包含一个引物组，可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保：（i）qPCR分析中的最佳退火温度为65°C（含2 mM Mg2+，不含DMSO）;（ii）除非另有说明，否则引物组可识别靶基因的所有已知转录本变体;（iii）只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

Preadipocytes are fibroblast-like precursor cells that can proliferate and differentiate into mature adipocytes. Preadipocytes play an important role in metabolic homeostasis and nutrient signaling. ScienCell's GeneQuery™ Human Preadipocyte Cell Biology qPCR Array Kit (GQH-PAD) is designed to facilitate gene expression profiling of 88 key genes involved in human preadipocyte cell biology, such as cell proliferation, differentiation, ECM formation and lipid metabolism. Genes implicated in obesity and liposarcoma are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:

· Preadipocyte cell markers: ADIPOQ, LEP, HOXC8/9, TBX1, TMEM26, UCP1, CIDEA, PRDM16, SLC7A10, P2RX5

· Proliferation: CCND1, PROKR1, LYRM1, FTO, SLC2A4, PDGFA, IL4, IGF1, FGF5, MAPK1/3, TNFSF10

· Differentiation and adipogenesis: PPARG, CEBPA/B, KLF2/5/15, SOCS1/3, INSR, DLK1, ADD1, SREBF1

· ECM and cytoskeleton: MMP1/2/3, FAP, DPP4, TGFB1, COL1A1/2, SMAD2/4

· Lipid metabolism: FABP5/7, LBP, LPL, PLIN2, ACSL1, LIPC

· Obesity: MC4R, UCP3, ADRB2/3, POMC, FFAR4, CARTPT

· Liposarcoma: FUS, MDM2, HMGA2, DDIT3, CDK4, EWSR1, DES, VIM

Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查询™人前脂肪细胞细胞生物学qPCR阵列板布局 *（8 个粗体和斜体控件）

注意：所有基因名称都遵循HGNC的符号

提示：基因选择可能会根据新的研究和开发进行更新

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