GK102 ScienCell 人类滋养层细胞生物学qPCR检测试剂盒，Human Trophoblast Cell Biology qPCR Array Kit

ScienCell 研究实验室（ScienCell）是一家生物技术公司，其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞，细胞培养基和试剂，培养基添加因子，细胞来源的RNA，cDNA和蛋白质。ScienCell的科学家们以改善生活质量，实现细胞的能力，打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥，公司成立于1999年。

北京作为ScienCell品牌的特约代理商，为客户提供，正规进口，保证原装正品，货期稳定，折扣好的服务标准。

GK102 ScienCell人类滋养层细胞生物学qPCR检测试剂盒，Human Trophoblast Cell Biology qPCR Array Kit，产品简介：

产品品牌： ScienCell

产品货号：GK102

产品名称：GK102 ScienCell人类滋养层细胞生物学qPCR检测试剂盒，Human Trophoblast Cell Biology qPCR Array Kit

产品规格：One 96-well plate, one 20ul reaction per well

北京裕恒丰科技有限公司是ScienCell中国区总代理，提供各种基因表达分析相关品，ScienCell细胞培养基、转染试剂、细胞检测试剂盒等细胞生物学产品。

GK102 ScienCell人类滋养层细胞生物学qPCR检测试剂盒，Human Trophoblast Cell Biology qPCR Array Kit， 产品说明：

滋养层细胞形成囊胚的外层，随后分化为中间滋养层、细胞滋养层和合体滋养层。滋养层在胚胎植入中起着核心作用，并与胎盘的母体蜕膜层相互作用。ScienCell 的 GeneQuery™ 人类滋养层细胞生物学 qPCR 阵列试剂盒 （GQH-TRO） 旨在促进参与人类滋养层发育、增殖和分化的 88 个关键基因的基因表达谱分析，以及滋养层细胞生物学中的重要途径，包括 LIF-STAT、FGF 和 NODAL/TGFB 信号通路。与妊娠滋养细胞疾病和绒毛膜癌有关的基因也包含在阵列中。下面显示了如何根据其功能对基因进行分组的简要示例：

滋养层细胞标志物：KRT7，GATA3，TFAP2C，ELF5，HLA-G，CGA，CGB3，CDX2，EOMES，ITGA1，VIM，POU5F1

开发和扩散：NANOG，SOX2，ESRRB，ASCL2，FLT1，HAND1，BHLHE40，GCM1，节点，PCSK6，FURIN

滋养层分化：TCF4，ASCL2，ID1 / 2，TFAP2C，HAND1，THBD，EIF3A，NCOA6，HSF1，GCM1，DLX3

LIF-STAT信令：IL6ST，LIFR，STAT1 / 3，SOCS3，TIMP1，ITGB3，JUNB

FGF 信令： FGF4， FGFR2， MAPK1/3， FRS2， PTPN11

节点/TGFB 信令：节点、ACVR2A/B、ACVR1B、SMAD2/3

妊娠滋养细胞疾病：INHA，LNPEP，GPC3，EGFR，ERBB2 / 3，PAM，CGB3，KRT18，PRPTJ，ALPP

绒毛膜癌：HOPX，HLA-G，GH2，CGA，LNPEP，CYP19A1，ERV3-1，CYP11A1，ABCG2

注意：所有基因名称都遵循人类基因组组织基因命名委员会（HGNC）的符号。

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪，每个孔包含一个引物组，可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保：（i）qPCR分析中的最佳退火温度为65°C（含2 mM Mg2+，不含DMSO）;（ii）除非另有说明，否则引物组可识别靶基因的所有已知转录本变体;（iii）只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

Trophoblast cells form the outer layer of the blastocyst and subsequently differentiate into the intermediate trophoblast, cytotrophoblast, and syncytiotrophoblast layers. Trophoblasts play a central role in embryo implantation and interact with the maternal decidual layer of the placenta. ScienCell's GeneQuery™ Human Trophoblast Cell Biology qPCR Array Kit (GQH-TRO) is designed to facilitate gene expression profiling of 88 key genes involved in human trophoblast development, proliferation and differentiation, as well as important pathways in trophoblast cell biology, including LIF-STAT, FGF and NODAL/TGFB signaling pathways. Genes implicated in gestational trophoblastic disease and choriocarcinoma are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:

· Trophoblast cell markers: KRT7, GATA3, TFAP2C, ELF5, HLA-G, CGA, CGB3, CDX2, EOMES, ITGA1, VIM, POU5F1

· Development and proliferation: NANOG, SOX2, ESRRB, ASCL2, FLT1, HAND1, BHLHE40, GCM1, NODAL, PCSK6, FURIN

· Trophoblast differentiation: TCF4, ASCL2, ID1/2, TFAP2C, HAND1, THBD, EIF3A, NCOA6, HSF1, GCM1, DLX3

· LIF-STAT signaling: IL6ST, LIFR, STAT1/3, SOCS3, TIMP1, ITGB3, JUNB

· FGF signaling: FGF4, FGFR2, MAPK1/3, FRS2, PTPN11

· NODAL/TGFB signaling: NODAL, ACVR2A/B, ACVR1B, SMAD2/3

· Gestational trophoblastic disease: INHA, LNPEP, GPC3, EGFR, ERBB2/3, PAM, CGB3, KRT18, PRPTJ, ALPP

· Choriocarcinoma: HOPX, HLA-G, GH2, CGA, LNPEP, CYP19A1, ERV3-1, CYP11A1, ABCG2

Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查询™人滋养层细胞生物学 qPCR 阵列板布局*（8 个粗体和斜体控件）

注意：所有基因名称都遵循HGNC的符号

提示：基因选择可能会根据新的研究和开发进行更新

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