ScienCellGK102人类滋养层细胞生物学qPCR检测的特点

2024-08-08

GK102 ScienCell 人类滋养层细胞生物学qPCR检测试剂盒,Human Trophoblast Cell Biology qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京作为ScienCell品牌的特约代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK102 ScienCell人类滋养层细胞生物学qPCR检测试剂盒,Human Trophoblast Cell Biology qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK102

产品名称:GK102 ScienCell人类滋养层细胞生物学qPCR检测试剂盒,Human Trophoblast Cell Biology qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

北京裕恒丰科技有限公司是ScienCell中国区总代理,提供各种基因表达分析相关品,ScienCell细胞培养基、转染试剂、细胞检测试剂盒等细胞生物学产品。

GK102 ScienCell人类滋养层细胞生物学qPCR检测试剂盒,Human Trophoblast Cell Biology qPCR Array Kit, 产品说明:

滋养层细胞形成囊胚的外层,随后分化为中间滋养层、细胞滋养层和合体滋养层。滋养层在胚胎植入中起着核心作用,并与胎盘的母体蜕膜层相互作用。ScienCell 的 GeneQuery™ 人类滋养层细胞生物学 qPCR 阵列试剂盒 (GQH-TRO) 旨在促进参与人类滋养层发育、增殖和分化的 88 个关键基因的基因表达谱分析,以及滋养层细胞生物学中的重要途径,包括 LIF-STAT、FGF 和 NODAL/TGFB 信号通路。与妊娠滋养细胞疾病和绒毛膜癌有关的基因也包含在阵列中。下面显示了如何根据其功能对基因进行分组的简要示例:

滋养层细胞标志物:KRT7,GATA3,TFAP2C,ELF5,HLA-G,CGA,CGB3,CDX2,EOMES,ITGA1,VIM,POU5F1

开发和扩散:NANOG,SOX2,ESRRB,ASCL2,FLT1,HAND1,BHLHE40,GCM1,节点,PCSK6,FURIN

滋养层分化:TCF4,ASCL2,ID1 / 2,TFAP2C,HAND1,THBD,EIF3A,NCOA6,HSF1,GCM1,DLX3

LIF-STAT信令:IL6ST,LIFR,STAT1 / 3,SOCS3,TIMP1,ITGB3,JUNB

FGF 信令: FGF4, FGFR2, MAPK1/3, FRS2, PTPN11

节点/TGFB 信令:节点、ACVR2A/B、ACVR1B、SMAD2/3

妊娠滋养细胞疾病:INHA,LNPEP,GPC3,EGFR,ERBB2 / 3,PAM,CGB3,KRT18,PRPTJ,ALPP

绒毛膜癌:HOPX,HLA-G,GH2,CGA,LNPEP,CYP19A1,ERV3-1,CYP11A1,ABCG2

注意:所有基因名称都遵循人类基因组组织基因命名委员会(HGNC)的符号。

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+,不含DMSO);(ii)除非另有说明,否则引物组可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

Trophoblast cells form the outer layer of the blastocyst and subsequently differentiate into the intermediate trophoblast, cytotrophoblast, and syncytiotrophoblast layers. Trophoblasts play a central role in embryo implantation and interact with the maternal decidual layer of the placenta. ScienCell's GeneQuery™ Human Trophoblast Cell Biology qPCR Array Kit (GQH-TRO) is designed to facilitate gene expression profiling of 88 key genes involved in human trophoblast development, proliferation and differentiation, as well as important pathways in trophoblast cell biology, including LIF-STAT, FGF and NODAL/TGFB signaling pathways. Genes implicated in gestational trophoblastic disease and choriocarcinoma are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:

· Trophoblast cell markers: KRT7, GATA3, TFAP2C, ELF5, HLA-G, CGA, CGB3, CDX2, EOMES, ITGA1, VIM, POU5F1

· Development and proliferation: NANOG, SOX2, ESRRB, ASCL2, FLT1, HAND1, BHLHE40, GCM1, NODAL, PCSK6, FURIN

· Trophoblast differentiation: TCF4, ASCL2, ID1/2, TFAP2C, HAND1, THBD, EIF3A, NCOA6, HSF1, GCM1, DLX3

· LIF-STAT signaling: IL6ST, LIFR, STAT1/3, SOCS3, TIMP1, ITGB3, JUNB

· FGF signaling: FGF4, FGFR2, MAPK1/3, FRS2, PTPN11

· NODAL/TGFB signaling: NODAL, ACVR2A/B, ACVR1B, SMAD2/3

· Gestational trophoblastic disease: INHA, LNPEP, GPC3, EGFR, ERBB2/3, PAM, CGB3, KRT18, PRPTJ, ALPP

· Choriocarcinoma: HOPX, HLA-G, GH2, CGA, LNPEP, CYP19A1, ERV3-1, CYP11A1, ABCG2

Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查询™人滋养层细胞生物学 qPCR 阵列板布局*(8 个粗体和斜体控件)

注意:所有基因名称都遵循HGNC的符号

1

2

3

4

5

6

7

8

9

10

11

12

A

ABCG2

BHLHE40

CYP19A1

ERV3-1

FURIN

HSF1

ITGB3

MAPK3

PAM

SLC22A4

TCF4

ACTB

B

ACVR1B

CCL2

DLX3

ERVW-1

GATA3

HSP90B1

JUNB

MGAT4A

PAPPA

SMAD2

TEAD3

GAPDH

C

ACVR2A

CD2AP

EGFR

ESRRB

GCM1

ID1

KHDC3L

NANOG

PCSK6

SMAD3

TFAP2A

LDHA

D

ACVR2B

CDKN1C

EIF3A

ETS2

GH2

ID2

KRT18

NCOA6

PLEKHH1

SMARCA4

TFAP2C

NONO

E

AFP

CDX2

ELF5

FGF4

GPC3

IGF2

KRT7

NLRP2

POU5F1

SOCS3

TFPI2

PPIH

F

ALPP

CGA

EOMES

FGFR2

HAND1

IL6ST

LIFR

NLRP5

PTPN11

SOX2

THBD

GDC

G

ALPPL2

CGB3

ERBB2

FLT1

HLA-G

INHA

LNPEP

NODAL

PTPRJ

STAT1

TIMP1

PPC

H

ASCL2

CYP11A1

ERBB3

FRS2

HOPX

ITGA1

MAPK1

NR0B1

SLC19A1

STAT3

VIM

NTC

提示:基因选择可能会根据新的研究和开发进行更新

ScienCell基因表达分析产品订购信息:

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GK003 人类细胞周期qPCR检测试剂盒,Human Cell Cycle qPCR Array Kit

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GK006 人类神经发育和再生qPCR检测试剂盒,Human Neural Development and Regeneration qPCR Array Kit

GK007 人类神经可塑性qPCR检测试剂盒,Human Neural Plasticity qPCR Array Kit

GK008 人类神经传递和膜转运qPCR检测试剂盒,Human Neural Transmission and Membrane Trafficking qPCR Array Kit

GK009 人类星型胶质细胞生物学qPCR检测试剂盒,Human Astrocyte Cell Biology qPCR Array Kit

GK010 人类黑素细胞发育和色素沉着qPCR检测试剂盒,Human Melanocyte Development & Pigmentation qPCR Array Kit

GK011 人类黑素细胞生物学qPCR检测试剂盒,Human Melanocyte Cell Biology qPCR Array Kit

GK013 人类星型胶质细胞转录因子qPCR检测试剂盒,Human Astrocyte Transcription Factors qPCR Array Kit

GK017 人血管生成qPCR检测试剂盒,Human Angiogenesis qPCR Array Kit

GK018 人内皮细胞表型异质性qPCR检测试剂盒,Human Endothelial Cell Phenotypic Heterogeneity qPCR Array Kit

GK019 人内皮细胞生物学qPCR检测试剂盒,Human Endothelial Cell Biology qPCR Array Kit

GK020 人B细胞受体信号通路qPCR检测试剂盒,Human B Cell Receptor Signaling Pathway qPCR Array Kit

GK021 人类T细胞受体信号通路qPCR检测试剂盒,Human T Cell Receptor Signaling Pathway qPCR Array Kit

GK022 人内吞作用qPCR检测试剂盒,Human Endocytosis qPCR Array Kit

GK023 人皮内细胞分化qPCR检测试剂盒,Human Endothelial Cell Differentiation qPCR Array Kit

GK024 人体剪切应力和机械传导qPCR检测试剂盒,Human Shear Stress and Mechanotransduction qPCR Array Kit

GK025 人类Notch信号通路qPCR检测试剂盒,uman Notch Signaling Pathway qPCR Array Kit

GK026 人类Wnt信号通路qPCR检测试剂盒,Human Wnt Signaling Pathway qPCR Array Kit

GK027 人类非经典Wnt信号通路qPCR检测试剂盒,Human Noncanonical Wnt Signaling Pathways qPCR Array Kit

GK028 人类Wnt信号靶基因qPCR检测试剂盒,Human Wnt Signaling Target Genes qPCR Array Kit

GK029 Human Tie2/Angpt信号qPCR检测试剂盒,Human Tie2/Angpt Signaling qPCR Array Kit

GK034 人肺纤维化qPCR检测试剂盒,Human Pulmonary Fibrosis qPCR Array Kit

GK035 人体缺氧反应qPCR检测试剂盒,Human Hypoxia Response qPCR Array Kit

GK036 人类HIF1信号响应qPCR检测试剂盒,Human HIF1 Signaling Response qPCR Array Kit

GK037 人巨噬细胞细胞生物学qPCR检测试剂盒,Human Macrophage Cell Biology qPCR Array Kit

GK038 人类吞噬作用qPCR检测试剂盒,Human Phagocytosis qPCR Array Kit

GK039 人类先天免疫反应qPCR检测试剂盒,Human Innate Immune Response qPCR Array Kit

GK040 人成纤维细胞生长因子信号通路qPCR检测试剂盒,Human Fibroblast Growth Factor Signaling Pathway qPCR Array Kit

GK041 人体皮肤伤口愈合qPCR检测试剂盒,Human Skin Wound Healing qPCR Array Kit

GK056 人体止血qPCR检测试剂盒,Human Hemostasis qPCR Array Kit

GK060 人类昼夜节律qPCR检测试剂盒,Human Circadian Rhythm qPCR Array Kit

GK061 人类DNA损伤感应qPCR检测试剂盒,Human DNA Damage Sensing qPCR Array Kit

GK062 人类细胞生长和分裂qPCR检测试剂盒,Human Cell Growth and Division qPCR Array Kit

GK063 人细胞周期蛋白和细胞周期蛋白依赖性激酶qPCR检测试剂盒,Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit

GK069 人类小胶质细胞生物学qPCR检测试剂盒,Human Microglia Cell Biology qPCR Array Kit

GK072 人类适应性免疫反应qPCR检测试剂盒,Human Adaptive Immune Response qPCR Array Kit

GK073 人类小胶质细胞Sensome qPCR检测试剂盒,Human Microglial Sensome qPCR Array Kit

GK074 人角质细胞生物学qPCR检测试剂盒,Human Keratocyte Biology qPCR Array

GK078 人类神经细胞生物学qPCR检测试剂盒,Human Neuronal Cell Biology qPCR Array Kit

GK079 人类软骨细胞生物学qPCR检测试剂盒,Human Chondrocyte Biology qPCR Array Kit

GK080 人成骨分化qPCR检测试剂盒,GeneQuery™ Human Osteogenic Differentiation qPCR Array Kit

GK081 人类周细胞生物学qPCR检测试剂盒,Human Pericyte Biology qPCR Array Kit

GK084 人体轴突导向qPCR检测试剂盒,Human Axon Guidance qPCR Array kit

GK087 人类染色质组织和重塑qPCR检测试剂盒,Human Chromatin Organization and Remodeling qPCR Array Kit

GK088 人VEGF信号通路qPCR检测试剂盒,Human VEGF Signaling Pathway qPCR Array Kit

GK089 人类Ephrin信号qPCR检测试剂盒,Human Ephrin Signaling qPCR Array Kit

GK090 人体细胞连接qPCR检测试剂盒,Human Cell Junctions qPCR Array Kit

GK091 人肝星状细胞生物学qPCR检测试剂盒,Human Hepatic Stellate Cell Biology qPCR Array Kit

GK092 人胰腺星状细胞生物学qPCR检测试剂盒,Human Pancreatic Stellate Cell Biology qPCR Array Kit

GK093 Human Renal Mesangial Cell Biology qPCR Array Kit,Human Renal Mesangial Cell Biology qPCR Array Kit

GK094 Human Mesenchymal Stem Cell Differentiation qPCR Array,Human Mesenchymal Stem Cell Differentiation qPCR Array Kit

GK095 人间充质至上皮细胞转化qPCR检测试剂盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit

GK096 人类雪旺细胞生物学qPCR检测试剂盒,Human Schwann Cell Biology qPCR Array Kit

GK097 人类平滑肌细胞生物学qPCR检测试剂盒,Human Smooth Muscle Cell Biology qPCR Array Kit

GK098 人类心脏肌细胞生物学qPCR检测试剂盒,Human Cardiac Myocyte Biology qPCR Array Kit

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GK102 人类滋养层细胞生物学qPCR检测试剂盒,Human Trophoblast Cell Biology qPCR Array Kit

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GK105 人类ECM降解qPCR检测试剂盒,Human ECM Degradation qPCR Array Kit

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GK114 人类小胶质细胞极化标记qPCR检测试剂盒,Human Microglial Polarization Markers qPCR Array Kit

GK115 人类骨髓谱系标记qPCR检测试剂盒,Human Myeloid Lineage Markers qPCR Array Kit

GK116 人B细胞和T细胞发育标记qPCR检测试剂盒,Human B and T Cells Development Markers qPCR Array Kit

GK117 人胸腺细胞发育标记qPCR检测试剂盒,Human Thymocyte Development Markers qPCR Array Kit

GK118 人类先天淋巴细胞发育标记qPCR检测试剂盒,Human Innate Lymphoid Cell Development Markers qPCR Array Kit

GK119 人类神经祖细胞标记qPCR检测试剂盒,Human Neural Progenitor Markers qPCR Array Kit

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GK123 人类生长因子qPCR检测试剂盒,Human Growth Factors qPCR Array Kit

GK124 人类转录因子qPCR检测试剂盒,Human Transcription Factors qPCR Array Kit

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GK126 人类细胞表面标记qPCR检测试剂盒,Human Cell Surface Markers qPCR Array Kit

GK127 人脑发育标记物qPCR检测试剂盒,Human Brain Development Markers qPCR Array Kit

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