ScienCellGK078人类神经细胞生物学qPCR检测试剂盒

2024-08-08

GK078 ScienCell 人类神经细胞生物学qPCR检测试剂盒,Human Neuronal Cell Biology qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京作为ScienCell品牌的代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK078 ScienCell人类神经细胞生物学qPCR检测试剂盒,Human Neuronal Cell Biology qPCR Array Kit,产品简介:

产品品牌:ScienCell

产品货号:GK078

产品名称:GK078 ScienCell人类神经细胞生物学qPCR检测试剂盒,Human Neuronal Cell Biology qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

GK078 ScienCell人类神经细胞生物学qPCR检测试剂盒,Human Neuronal Cell Biology qPCR Array Kit, 产品说明:

ScienCell的GeneQuery™人类神经元细胞生物学qPCR阵列(GQH-NEU)分析了88个参与正常神经元功能的关键基因。 神经元负责通过电势在全身处理和传递信息。 它们是中枢神经系统的核心组成部分,专门的神经元是感觉和运动单位的关键元素。 以下是如何根据其在神经元细胞生物学中的功能对所包含的基因进行分组的简要示例:

神经传递:ABAT、GABRB1、GRM3、SLC32A1、VAMP2

轴突引导:EFNA2、ROBO1、NTN1、NRP1、SLIT1

突触形成和维持:DLG2,NLGN3,SYN1,SYP,DLG4

神经突生长:GLDN,GPRIN1,NRCAM,RTN4,SEMA3G

差异化: BMP2, EVX1, GNL3, SMARCA4, PROM1

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可识别并有效扩增特定靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+且不含DMSO);(ii)除非另有说明,否则引物集可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Neuronal Cell Biology qPCR Array (GQH-NEU) profiles 88 key genes involved in normal neuron function. Neurons are responsible for processing and transmitting information throughout the body via electric potentials. They are a core component of the central nervous system and specialized neurons are key elements of the sensory and motor units. Below are brief examples of how included genes may be grouped according to their function in neuronal cell biology:

· Neurotransmission: ABAT, GABRB1, GRM3, SLC32A1, VAMP2

· Axonal guidance: EFNA2, ROBO1, NTN1, NRP1, SLIT1

· Synapse formation and maintenance: DLG2, NLGN3, SYN1, SYP, DLG4

· Neurite outgrowth: GLDN, GPRIN1, NRCAM, RTN4, SEMA3G

· Differentiation: BMP2, EVX1, GNL3, SMARCA4, PROM1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人类神经元细胞生物学 qPCR 阵列板布局*(8 个粗体和斜体对照)

1

2

3

4

5

6

7

8

9

10

11

12

A

ABAT

EFNA2

EPHA7

GABRB1

GRM7

NFASC

NRP2

PLCG1

ROBO2

SEMA3F

SYP

β-actin

B

ABCG2

EFNA5

EPHB1

GAD1

HRAS

NGF

NTF3

PLCG2

RTN4

SEMA3G

TUBB3

GAPDH

C

BDNF

EFNB1

EPHB2

GAD2

ITGB1

NGRN

NTF4

PROM1

RTN4R

SLC32A1

UNC5A

LDHA

D

BMP2

EFNB2

EVX1

GFAP

KRAS

NLGN3

NTN1

RAB11A

S100B

SLC6A1

UNC5B

NONO

E

CLTA

ENO2

FZD1

GLDN

MAP2

NOTCH1

NTRK1

RAB5A

SEMA3A

SLIT1

UNC5C

PPIH

F

DCC

EPHA1

GABBR1

GNL3

NEDD4L

NOTCH2

NTRK2

RAB7A

SEMA3C

SLIT2

VAMP2

GDC

G

DLG2

EPHA2

GABBR2

GPRIN1

NES

NRCAM

NTRK3

RBFOX3

SEMA3D

SMARCA4

VAV2

PPC

H

DYNC1H1

EPHA5

GABRA2

GRM3

NEUROD1

NRP1

PDGFA

ROBO1

SEMA3E

SYN1

VAV3

NTC

提示:基因选择可能会根据新的研究和开发进行更新

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