ScienCellGK056人体止血qPCR检测试剂盒

2024-08-08

GK056 ScienCell 人体止血qPCR检测试剂盒,Human Hemostasis qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京作为ScienCell品牌的代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK056 ScienCell人体止血qPCR检测试剂盒,Human Hemostasis qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK056

产品名称:GK056 ScienCell人体止血qPCR检测试剂盒,Human Hemostasis qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

GK056 ScienCell人体止血qPCR检测试剂盒,Human Hemostasis qPCR Array Kit, 产品说明:

ScienCell的GeneQuery™人类止血qPCR阵列试剂盒(GQH-HEM)分析了88个参与止血的关键基因。 受伤后,止血通常包括血管收缩以防止血液流向受感染区域,形成临时栓子以抑制出血,然后凝血或血液凝固。 止血障碍的例子是血友病、血栓形成和栓塞。 如何根据其在止血中的功能对所包含的基因进行分组的简要示例如下所示:

血小板活化: F2R, FCER1G, FYN, PLA2G4A, PIK3CB

凝血: F8, PF4, 平台, THBD, COL4A1

凝血: A2M, F7, 普劳尔, C5AR1, C7

血友病: F2, F3, VWF, IL10, F5

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可识别并有效扩增特定靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+且不含DMSO);(ii)除非另有说明,否则引物集可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Hemostasis qPCR Array Kit (GQH-HEM) profiles 88 key genes involved in stopping bleeding. Upon wounding, hemostasis generally involves vasoconstriction to prevent blood flow to the afflicted area, forming a temporary plug to inhibit bleeding, and then coagulation or blood clotting. Examples of disorders in hemostasis are hemophilia, thrombosis, and embolisms. Brief examples of how included genes may be grouped according to their function in hemostasis are shown below:

· Platelet activation: F2R, FCER1G, FYN, PLA2G4A, PIK3CB

· Clotting: F8, PF4, PLAT, THBD, COL4A1

· Coagulation: A2M, F7, PLAUR, C5AR1, C7

· Hemophilia: F2, F3, VWF, IL10, F5

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

基因查询™人类止血 qPCR 阵列板布局*(8 个粗体和斜体对照)

1

2

3

4

5

6

7

8

9

10

11

12

A

A2M

APP

F3

FYN

IL3

JAK3

PFN1

PLAUR

PSAP

SOS1

TMSB4X

β-actin

B

ACTN1

C5AR1

F5

GNAQ

IL5

LAT

PIK3CB

PLCG2

PTK2

SPARC

TUBA4A

GAPDH

C

ACTN2

C6

F7

IGF1

IL6

LYN

PIK3CD

PLEK

SELP

SRGN

VAV1

LDHA

D

ADRA2A

C7

F8

IGF2

ITGA2B

MPL

PIK3R1

PLG

SEPP1

TGFB1

VAV2

NONO

E

AKT2

CLU

FCER1G

IL10

ITGB3

MTCH1

PIKFYVE

PRF1

SERPINA1

THBD

VAV3

PPIH

F

AKT3

COL4A1

FIGF

IL2

ITPR1

P2RY12

PLA2G4A

PRKCA

SERPINE1

THBS1

VCL

GDC

G

APBB1IP

F2

FLNA

IL2RA

ITPR2

PECAM1

PLAT

PRKCE

SFTPA1

TIMP1

VEGFA

PPC

H

APOA1

F2R

FN1

IL2RB

JAK2

PF4

PLAU

PRKCQ

SOD1

TLN1

VWF

NTC

提示:基因选择可能会根据新的研究和开发进行更新

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