Alpco 80-CPTHU-E01.1 C-Peptide ELISA | 人C肽酶免试剂盒,96 wells
Alpco公司从成立初期就致力于生物医药领域各种免疫检测试剂盒的研发与生产,力求为广大客户提供高质量的免疫检测产品。 Alpco产品种类多样,可测样品来源包括人、大/小鼠、豚鼠、兔、猪、羊、狗、猴等,涉及神经生物学、骨代谢、心血管疾病和氧化应激、细胞因子与信号转导、糖尿病和肥胖症、内分泌学、甲状腺功能、肠胃病学、免疫学、生殖激素、抗体和抗原、分子诊断等多个研究领域,尤以糖尿病和肥胖症研究领域见长。
我们作为Alpco公司的代理商为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。
Alpco 80-CPTHU-E01.1 C-Peptide ELISA,人C肽酶免试剂盒,96 well,产品简介:
产品品牌:Alpco
产品名称:人C肽酶免试剂盒
英文名称:Alpco 80-CPTHU-E01.1 C-Peptide ELISA
产品货号:80-CPTHU-E01.1
产品规格:96 well
Alpco 80-CPTHU-E01.1 C-Peptide ELISA,人C肽酶免试剂盒,96 well,产品说明检测原理:
ALPCO C-肽ELISA是一个夹层式免疫检测试剂盒。96孔微孔板涂有一层C-肽特异性单克隆抗体。将标准物,对照品和样品一并添加到微型板小孔中与检测缓冲液混合。然后将微孔板放在摇床上室温温育1小时,700-900转/分钟。*次温育结束,用清洗缓冲液清洗小孔并烘干。添加结合物(配制方法参照说明书),微孔板放在摇床上第二次室温温育1小时,700-900转/分钟。添加TMB作用底物,放在微孔板摇床上室温温育15分钟,700-900转/分钟。第三次温育结束,添加反应终止液,调节分光光度计到450nm测定OD值。由于发光强度与样品中C-肽含量成比例,因此可以根据OD值确定C-肽含量。
§ 检测过程:各添加25μl标准物,对照物和样品→加50µl检测缓冲液→微孔板摇床室温温育1小时,700-900rpm→清洗缓冲液清洗6次→加100µl结合物(配制方法参照说明书)→微孔板摇床室温温育1小时,700-900rpm→清洗缓冲液清洗6次→添加100µl TMB作用底物→微孔板摇床室温温育15分钟,700-900rpm→添加100µl反应终止液→分光光度计450nm读数
ALPCO C-peptide ELISA kit英文简述:
The ALPCO C-peptide ELISA kit is a FDA registered in vitro diagnostic tool for the quantification of human C-peptide in a clinical setting or research laboratory. Utilizing a dual-monoclonal antibody sandwich ELISA format, bi-level control set, and 96-well microplate comprising removable strips, a single C-peptide ELISA kit has the performance characteristics and flexibility necessary to confidently measure up to 40 samples in duplicate. The shelf-life of the components of the C-Peptide ELISA and the resealable microplate pouch allow for convenient storage for future use if the entire kit is not needed at one time.
INTENDED USE:The ALPCO C-peptide ELISA is designed for the quantitative determination of C-peptide in human serum and plasma.
PRINCIPLE OF THE ASSAY:The ALPCO C-peptide ELISA is a sandwich type immunoassay. The 96-well microplate is coated with a monoclonal antibody specific for C-peptide. The standards, controls, and samples are added to the microplate wells with the Assay Buffer. The microplate is then incubated at room temperature on a microplate shaker at 700-900 rpm. After the first incubation is complete, the wells are washed with Wash Buffer and blotted dry. The Conjugate is then added, and the microplate is incubated a second time on a microplate shaker at 700-900 rpm, washed, and blotted dry. TMB Substrate is added, and the microplate is incubated a third time at room temperature on a microplate shaker at 700-900 rpm. Once the third incubation is complete, Stop Solution is added, and the optical density (OD) is measured by a spectrophotometer at 450 nm. The intensity of the color generated is directly proportional to the amount of C-peptide in the sample.
Alpco品牌其它部分产品货号:
产品名称 | 货号 | 样本容量 | 规格 |
Insulin ELISA | 80-INSHU-E01 | 25µl | 96T/盒 |
Insulin Ultrasensitive ELISA | 80-INSHUU-E01 | 25µl | 96T/盒 |
Insulin (Synthetic) ELISA | 80-SINHU-E01 | 25µl | 96T/盒 |
Insulin (Mouse) ELISA | 80-INSMS-E01 | 10µl | 96T/盒 |
Insulin (Mouse) High Range ELISA | 80-INSMSH-E01 | 5µl | 96T/盒 |
Insulin (Mouse) Ultrasensitive ELISA(5µl) | 80-INSMSU-E01 | 5µl | 96T/盒 |
Insulin (Mouse) Ultrasensitive ELISA(25µl) | 80-INSMSU-E01 | 25µl | 96T/盒 |
Insulin (Rat) ELISA | 80-INSRT-E01 | 10µl | 96T/盒 |
Insulin (Rat) High Range ELISA | 80-INSRTH-E01 | 5µl | 96T/盒 |
Insulin (Rat) Ultrasensitive ELISA(5µl) | 80-INSRTU-E01 | 5µl | 96T/盒 |
Insulin (Rat) Ultrasensitive ELISA(25µl) | 80-INSRTU-E01 | 25µl | 96T/盒 |
Insulin (Bovine) ELISA | 80-INSBO-E01 | 25µl | 96T/盒 |
Insulin (Ovine) ELISA | 80-INSOV-E01 | 25µl | 96T/盒 |
Insulin (Porcine/Canine) ELISA | 80-INSPO-E01 | 25µl | 96T/盒 |
C-Peptide ELISA | 80-CPTHU-E01.1 | 25µl | 96T/盒 |
C-Peptide (Mouse) ELISA | 80-CPTMS-E01 | 10µl | 96T/盒 |
C-Peptide (Rat) ELISA | 80-CPTRT-E01 | 10µl | 96T/盒 |
Proinsulin (Mouse) ELISA | 80-PINMS-E01 | 10µl | 96T/盒 |
Proinsulin ELISA | 80-PINHU-E01 | 50µl | 96T/盒 |
Proinsulin (Rat) ELISA | 80-PINRT-E01 | 10µl | 96T/盒 |
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