BIO-RAD MCA596GT Mouse anti Human CD14,Mouse anti Human CD14 antibody, clone UCHM1-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

BIO-RAD MCA596GT Mouse anti Human CD14,Mouse anti Human CD14 antibody, clone UCHM1

2024-10-22

Mouse anti Human CD14 antibody, clone UCHM1

货号：MCA596GT,MCA596GA,MCA596G

规格：25 µg,0.1 mg,0.2 mg

价格：935,2023,3400

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD14

物种：人

宿主：小鼠

抗体亚型：IgG2a

克隆号：UCHM1

荧光染料：Purified

产品介绍
Mouse anti Human CD14 antibody, clone UCHM1recognizes a cell surface antigen of ~55 kDa, known as CD14.The CD14 molecule is found predominantly on monocytes and macrophages in flow cytometry, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology the CD14 molecule is found to be present on Langerhans cells, follicular dendritic cells, histiocytes and high endothelial venules. Antibodies to the CD14 molecule are known to induce oxidative burst formation. In tonsil tissue sections UCHM1 gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells. Skin Langerhans cells are always negative (Hogget al.1984). UCHM1 also reacts with Kupffer cells and sinus lining cells on the liver.
产品详情
Target Species	Human
Product Form	Purified IgG - liquid
Preparation	MCA596G, MCA596GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant MCA596GT: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09%Sodium Azide
Carrier Free	Yes
Immunogen	Human Thymocytes followed by peripheral blood mononuclear cells.
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells from the NS1-Ag4/1 mouse myeloma line.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Hogg, N. & Horton, M.A. (1987) Myeloid antigens: new and previously defined clustersin Leucocyte Typing III White Cell differentiation antigens. Edited by McMichael, A.J.,et al.2.Yoshino, N.et al.(2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.Exp Anim. 49 (2): 97-110.3.Jonker, M.et al. (1989) Reactivity of mAb specific for human CD markers with Rhesus monkey leucocytes.Leucocyte Typing IV. Oxford University Press p 1058-63.4.Hogg, N.et al.(1984) Monoclonal antibodies specific for human monocytes, granulocytes and endothelium.Immunology. 53 (4): 753-67.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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BIO-RAD MCA596GT Mouse anti Human CD14,Mouse anti Human CD14 antibody, clone UCHM1

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