BIO-RAD MCA4754A647 Mouse anti Rat CD63:Alexa Fluor®647,Mouse anti Rat CD63:Alexa Fluor®647

2024-10-22

Mouse anti Rat CD63:Alexa Fluor®647

货号:MCA4754A647

规格:100 Tests/1ml

价格:3596

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:CD63

物种:大鼠

宿主:小鼠

抗体亚型:IgG1, kappa

荧光染料:Alexa Fluor 488

产品介绍
Mouse anti Rat CD63 antibody, clone AD1recognizes rat CD63, an intracellular type-III lysosomal glycoprotein and member of the tetraspanin (TM4SF) family, found on activated platelets and resident macrophages. Rat CD63 is expressed on mast cells in tissues and can be induced on other cell typesin vitro(Nishikataet al.1992).Mouse anti Rat CD63 antibody, clone AD1 partially inhibits IgE-mediated histamine release and binding competition assays indicate that it is sterically situated close to the high-affinity IgE receptor on rat basophilic leukemia (RBL-2H3) cells (Kitaniet al.1991).
产品详情
Target SpeciesRat
Product FormPurified IgG conjugated to Alexa Fluor® 647 - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
ImmunogenRBL-2H3 rat basophilic leukaemia cell line.
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Approx. Protein ConcentrationsIgG concentration 0.05 mg/ml
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
Alexa Fluor®647650665
AcknowledgementsThis product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
文献
1.Kitani, S.et al.(1991) A cell surface glycoprotein of rat basophilic leukemia cells close to the high affinity IgE receptor (Fc epsilon RI). Similarity to human melanoma differentiation antigen ME491.J Biol Chem. 266 (3): 1903-9.

技术参数

产品应用 Flow

产品类型 单克隆抗体

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