BIO-RAD MCA4739P Mouse anti Rabbit GAPDH:HRP,Mouse anti Rabbit GAPDH:HRP

2024-10-22

Mouse anti Rabbit GAPDH:HRP

货号:MCA4739P

规格:0.1 mg

价格:3247

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:GAPDH

物种:兔

宿主:小鼠

抗体亚型:IgG1, kappa

荧光染料:HRP

产品介绍
Mouse anti Rabbit GAPDH antibody, clone 6C5recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a ~36 kDa multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism.GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death. A further report has shown that GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin (Haraet al.2006).
产品详情
Target SpeciesRabbit
Product FormPurified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.01% Thiomersal
ImmunogenRabbit muscle GAPDH.
Approx. Protein ConcentrationsIgG concentration 1.0mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Latasa, M.U.et al.(2010) Oral methylthioadenosine administration attenuates fibrosis and chronic liver disease progression in Mdr2-/- mice.PLoS One. 5: e15690.2.Haller, S.et al.(2012) Expression profiles of metabolic enzymes and drug transporters in the liver and along the intestine of beagle dogs.Drug Metab Dispos. 40 (8): 1603-10.3.Zizza, P.et al.(2012) Phospholipase A2IVα regulates phagocytosis independent of its enzymatic activity.J Biol Chem. 287: 16849-59.4.Zschemisch, N.H.et al.(2012) Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.BMC Immunol. 13: 60.

技术参数

产品应用 ELISA;FN

产品类型 单克隆抗体

推荐用于 ELISA;FN

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