mouse anti human CD45RO:StarBright Violet 515

货号：MCA461SBV515

规格：100 Tests/0.5ml

价格：3553

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD45RO

物种：人

宿主：小鼠

抗体亚型：IgG2a

克隆号：UCHL1

荧光染料：StarBright Violet 515

类型:	单抗	同型对照：	IgG2a
浓度：		用法：	5 µL/test(Flow)
免疫原性：	Human IL-2 dependent T cells

Mouse anti Human CD45RO monoclonal antibody, clone UCHL1recognizes the low molecular weight isoform (180 kDa) of the leucocyte common antigen (LCA). The antigen is expressed by a functional subset of T cells with memory phenotype.In peripheral blood Mouse anti Human CD45RO, clone UCHL1 stains 40-80% of lymphocytes and all monocytes and granulocytes.

数据

Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488) and SBV515 conjugated Mouse anti Human CD45RO (MCA461SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on theZE5 Cell Analyzer.

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参考文献：

1. Smith, S.H.et al. (1986) Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1.Immunology 58: 63-70.2. Norton, A.J.et al.(1986) Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues.J Clin Pathol. 39 (4): 399-405.3. Beverley, P.C.L.et al. (1986) T-cell subsets and function. Progress in Immunology VI. Cinader, B., Miller, G.G., eds.Academic Press Orlando pp 941-948.4. Beverley, P.C. (1987) Human T cell subsets.Immunol Lett. 14 (4): 263-7.5. Terry. L.A.et al. (1987) Phenotypic heterogeneity of the CD4+ and CD8+ subsets.Leucocyte Typing III. McMichael, A.J., Beverley, P.C.L.et al. eds. University Press. pp 225-7.6. Akbar, A.N.et al.(1988) Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells.J Immunol. 140 (7): 2171-8.7. Terry, L.A.et al.(1988) The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte-common antigen, CD45.Immunology. 64 (2): 331-6.8. Beverley, P.C.L.et al. (1988) Phenotypic diversity of the CD45 antigen and its relation to function.Immunology, Suppl. 1: 3-5.9. Merkenschlager, M.et al.(1988) Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation.Eur J Immunol. 18 (11): 1653-61.10. Cavers, M.et al.(2002) Differential expression of beta1 and beta2 integrins and L-selectin on CD4+ and CD8+ T lymphocytes in human blood: comparative analysis between isolated cells, whole blood samples and cryopreserved preparations.Clin Exp Immunol. 127: 60-5.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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