Bio-Rad Rat anti mouse IgG3 HEAVY chain:Biotin MCA423B,Rat anti mouse IgG3 HEAVY chain:Biotin

2024-10-22

Rat anti mouse IgG3 HEAVY chain:Biotin

货号:MCA423B

规格:0.5 mg

价格:4165

产品类型:二抗

品牌:Bio-Rad

抗原:IgG3

物种:小鼠

宿主:大鼠

抗体亚型:IgM

克隆号:LO-MG3-7

荧光染料:Biotin

产品介绍
Rat anti Mouse IgG3 Heavy Chain antibody, clone LO-MG3-7recognizes the gamma 3 heavy chain of mouse IgG and does not cross react with other subclasses of murine IgG.
产品详情
Target SpeciesMouse
Product FormPurified IgM conjugated to Biotin - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers<0.1% Sodium Azide (NaN3)50% Glycerol
ImmunogenPolyclonal and monoclonal mouse IgG3.
Approx. Protein ConcentrationsIgM concentration 1 mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA0.5ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
文献
1.Bazin, H. (1982) Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line.Prot Biol Fluids. Peters Ed: 29th Colloquim (1981). Pergamon Press, Oxford and N.Y., pp 615-8.2.Bazin, H.et al.(1984) Rat monoclonal antibodies. I. Rapid purification fromin vitroculture supernatants.J Immunol Methods. 66 (2): 261-9.3.Bazin, H.et al.(1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.J Immunol Methods. 71 (1): 9-16.4.Zivkovic, I.et al.(2012) Induction of decreased fecundity by tetanus toxoid hyper-immunization in C57BL/6 mice depends on the applied adjuvant.Innate Immun. 18: 333-42.

技术参数

产品应用 ELISA

产品类型 Monoclonal Antibody

推荐用于 ELISA

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