Bio-Rad Rat anti MBP (aa82-87) MCA409S,Rat anti MBP (aa82-87)-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad Rat anti MBP (aa82-87) MCA409S,Rat anti MBP (aa82-87)

2024-10-22

Rat anti MBP (aa82-87)

货号： MCA409S

规格：2 ml

价格：3358

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：MBP

物种：Bovine

宿主：大鼠

抗体亚型：IgG2a

克隆号：12

荧光染料：Purified

产品介绍
Rat anti MBP antibody, clone 12recognizes myelin basic protein from a wide range of species. Rat anti MBP antibody, clone 12 reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well with Rat anti MBP antibody, clone 12, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV).Rat anti MBP antibody, clone 12 has been reported as being suitable for use in western blotting (Glynnet al.1987).
产品详情
Target Species	Bovine
Product Form	Tissue Culture Supernatant - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution	0.1M TRIS
Immunogen	Bovine MBP.
Fusion Partners	Spleen cells from an immunised outbred rat were fused with cells of the mouse NS0 myeloma cell line.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Preservative Stabilisers	0.1%Sodium Azide
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Groome, N.P.et al.(1986) Region-specific immunoassays for human myelin basic protein.J Neuroimmunol. 12 (4): 253-64.2.Glynn, P.et al.(1987) Basic protein dissociating from myelin membranes at physiological ionic strength and pH is cleaved into three major fragments.J Neurochem. 48 (3): 752-9.3.Hruby, S.et al.(1987) Monoclonal antibodies reactive with myelin basic protein.Mol Immunol. 24 (12): 1359-64.4.Homchaudhuri Let al.(2009) Influence of membrane surface charge and post-translational modifications to myelin basic protein on its ability to tether the Fyn-SH3 domain to a membranein vitro.Biochemistry. 48 (11): 2385-93.5.Pohl, H.B.et al.(2011) Genetically induced adult oligodendrocyte cell death is associated with poor myelin clearance, reduced remyelination, and axonal damage.J Neurosci. 31 (3): 1069-80.

技术参数

产品应用 ELISA Immunofluorescence Radioimmunoassays Western Blotting

产品类型 Monoclonal Antibody

推荐用于 ELISA Immunofluorescence Radioimmunoassays Western Blotting

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Bio-Rad Rat anti MBP (aa82-87) MCA409S,Rat anti MBP (aa82-87)

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