BIO-RAD MCA470 Mouse anti Chicken Troponin T,Mouse anti Chicken Troponin T

2024-10-22

Mouse anti Chicken Troponin T

货号:MCA470

规格:0.25 mg

价格:3162

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Troponin T

物种:其它

宿主:小鼠

抗体亚型:IgG1, kappa

荧光染料:其它

产品介绍
Mouse anti Chicken Troponin T antibody, clone T1/61recognizes chicken Troponin T, fast skeletal muscle isoforms, also known as fast muscle Troponin T. Troponin T is a 262 amino acid tropomyosin binding component of the troponin complex with a predicted molecular mass of ~31 kDa.Four isoforms of Troponin T generated by alternative splicing have been described. Mouse anti Chicken Troponin T antibody, clone T1/61 recognizes the canonical isoform 1, binding to othe potential isoforms has not been evaluated (UniProt : P12620).
产品详情
Target SpeciesRat
Product FormPurified IgG - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
ImmunogenTroponin T, purified from chicken breast muscle
Fusion PartnersSpleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Preservative Stabilisers0.09%Sodium Azide
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry1/50
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.(2011) Human Protein Atlas: CAB000472Human Protein Atlas: CAB0004722.Bird, I.M.et al.(1985) Identification of multiple variants of fast muscle troponin T in the chicken using monoclonal antibodies.Eur J Biochem. 150 (3): 517-25.3.Watkins, H.et al.(1996) Expression and functional assessment of a truncated cardiac troponin T that causes hypertrophic cardiomyopathy. Evidence for a dominant negative action.J Clin Invest. 98: 2456-61.4.Marian, A.J.et al.(1997) Expression of a mutant (Arg92Gln) human cardiac troponin T, known to cause hypertrophic cardiomyopathy, impairs adult cardiac myocyte contractility.Circ Res. 81: 76-85.5.Dunnick, J.K.et al.(2004) Cardiac damage in rodents after exposure to bis(2-chloroethoxy)methane.Toxicol Pathol. 32: 309-17.

技术参数

产品应用 Flow

产品类型 单克隆抗体

推荐用于 Flow

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