Bio-Rad mouse anti V5-TAG:FITC MCA2894F,mouse anti V5-TAG:FITC-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad mouse anti V5-TAG:FITC MCA2894F,mouse anti V5-TAG:FITC

2024-10-22

mouse anti V5-TAG:FITC

货号：MCA2894F

规格：0.1 mg

价格：3290

产品类型：流式抗体

品牌：Bio-Rad

物种：Virus

宿主：小鼠

抗体亚型：IgG1

克隆号：SV5-Pk4

荧光染料：FITC

产品介绍
Mouse anti V5-Tag antibody, clone SV5-Pk4recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Mouse anti V5-Tag antibody, clone SV5-Pk4 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys proile pro asn pro leu leu gly leu aspser thr. (The 9 amino acid epitope is underlined).
产品详情
Target Species	Viral
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Approx. Protein Concentrations	IgG concentration 0.05 mg/ml
Immunogen	A recombinant peptide corresponding to the PK tag fused to a class II - restricted T helper cell epitope.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Acknowledgements	This product is manufactued under an exclusive license from the University of St. Andrews, UK.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
文献
1.Dunn, C.et al.(1999) Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag.J Immunol Methods. 224 (1-2): 141-50.

技术参数

产品应用 Flow;WB;IF

产品类型单克隆抗体

推荐用于 Flow;WB;IF

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Bio-Rad mouse anti V5-TAG:FITC MCA2894F,mouse anti V5-TAG:FITC

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