BIO-RAD MCA2747F Rat anti Mouse CD83 antibody, clone 3D11,Rat anti Mouse CD83 antibody, clone 3D11

2024-10-22

Rat anti Mouse CD83 antibody, clone 3D11

货号:MCA2747F

规格:0.1 mg

价格:2550

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD83

物种:小鼠

宿主:大鼠

抗体亚型:IgG2a

克隆号:3D11

荧光染料:FITC

产品介绍
Rat anti Mouse CD83 antibody, clone 3D11recognizes murine CD83, a 45 kDa type 1 glyoprotein belonging to the immunoglobulin superfamily. Originally described as a maturation marker for dendritic cells, CD83 plays a role in the regulation of antigen presentation, thymic T-cell maturation, B-cell function and peripheral T-cell activation. Activated dendritic cells and B-cells release a soluble form of CD83 that is reported to have an immunosuppressive effect, suggesting a therapeutic role for sCD83 in the regulation of immune disorders.
产品详情
Target SpeciesMouse
Product FormPurified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Carrier FreeYes
Preservative Stabilisers0.09% Sodium Azide (NaN3)1%Bovine Serum Albumin
Approx. Protein ConcentrationsIgG concentration 0.1 mg/ml
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
FITC490525
Fusion PartnersSpleen cells from immunised rats were fused with cells of the P3X63.Ag8.653 mouse myeloma cell line.
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat1/2Pack Size: 0.1 mgNeatPack Size: 25 µg
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
同型对照
DescriptionProduct CodeApplicationsPack Size
Rat IgG2a Negative Control:FITCMCA1212FF100 Tests
文献
1.Fujimoto, Y. & Tedder, T.F. (2006) CD83: a regulatory molecule of the immune system with great potential for therapeutic application.J Med Dent Sci. 53 (2): 85-91.2.He, Y.T.et al.(2016)In vitrogeneration of cytotoxic T lymphocyte response using dendritic cell immunotherapy in osteosarcoma.Oncol Lett. 12 (2): 1101-6.3.El-Ashmawy, N.E.et al.(2015)In vitroandin vivostudies of the immunomodulatory effect ofEchinacea purpureaon dendritic cells.Journal of Genetic Engineering and Biotechnology. 13 (2): 185-92.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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