Bio-Rad mouse anti PIG CD117:Alexa Fluor 488 MCA2598A488,mouse anti PIG CD117:Alexa Fluor 488-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad mouse anti PIG CD117:Alexa Fluor 488 MCA2598A488,mouse anti PIG CD117:Alexa Fluor 488

2024-10-22

mouse anti PIG CD117:Alexa Fluor 488

货号：MCA2598A488

规格：100 Tests/1ml

价格：4165

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD117

物种：pig

宿主：小鼠

抗体亚型：IgG1

克隆号：2B8/BM

荧光染料：Alexa Fluor 488

产品介绍
Mouse anti Pig CD117, clone 2B8/BMis specific for porcine CD117, also known as c-kit, a 155 kDa type I transmembrane protein with protein tyrosine kinase activity, which plays an important role in early hematopoiesis (Perezet al.2007).Hematopoietic stem cells (HSC) of bone marrow have multilineage differentiation potential and an extensive capacity for self-renewal. The majority of adult bone marrow hematopoietic progenitor cells are CD117+ and have been used successfully in xenograft transplantation models for long term survival of grafts, without symptoms of graft-versus-host disease (GVHD).Mouse anti pig CD117, clone 2B8/BM recognizes CD117 on a small subset of porcine bone marrow progenitor cells and therefore provides an alternative tool from the previously used c-kit ligand stem cell factor, for the isolation and enrichment of porcine stem cells.
产品详情
Target Species	Pig
Product Form	Purified IgG conjugated to Alexa Fluor® 488 - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Fusion Partners	Spleen cells from immunized Balb/c mouse were fused with cells of the Sp2/0 myeloma cell line.
Buffer Solution	Phosphate buffered saline
Immunogen	Mouse TREM-1 Fc fusion protein.
Approx. Protein Concentrations	IgG concentration 0.05mg/ml
Preservative Stabilisers	0.09%Sodium Azide1%Bovine Serum Albumin
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
Alexa Fluor®488	495	519
Acknowledgements	This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
文献
1.Wehman, B.et al.(2017) Cardiac Progenitor Cells Enhance Neonatal Right Ventricular Function After Pulmonary Artery Banding.Ann Thorac Surg. 104 (6): 2045-53.2.Pérez Cet al.(2007) Characterisation of porcine bone marrow progenitor cells identified by the anti-c-kit (CD117) monoclonal antibody 2B8/BM.J Immunol Methods. 321 (1-2): 70-9.3.Hatzistergos, K.E.et al.(2010) Bone marrow mesenchymal stem cells stimulate cardiac stem cell proliferation and differentiation.Circ Res. 107: 913-22.4.Escalona, Z.et al.(2014) Molecular characterization and expression of porcine Siglec-5.Dev Comp Immunol. 44 (1): 206-16.

技术参数

产品应用 Flow

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Bio-Rad mouse anti PIG CD117:Alexa Fluor 488 MCA2598A488,mouse anti PIG CD117:Alexa Fluor 488

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