Bio-Rad mouse anti SUPEROXIDE DISMUTASE (Cu-Zn),mouse anti SUPEROXIDE DISMUTASE (Cu-Zn)

2024-10-22

mouse anti SUPEROXIDE DISMUTASE (Cu-Zn)

货号:MCA2595

规格:0.1 mg

价格:2380

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原: superoxide dismutase

物种:Bovine

宿主:小鼠

抗体亚型:IgG1

克隆号:2F5

荧光染料:Purified

产品介绍
Mouse anti Bovine superoxide dismutase (Cu/Zn) antibody, clone 2F5recognizes bovine superoxide dismutase (Cu/Zn), also known by it's gene name SOD1. Bovine superoxide dismutase (Cu/Zn) is a 152 amino acid ~15kDa cytoplasmic protein involved in the removal of free radicals from the cytosol. Superoxide dismutases are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. As such, they are an important antioxidant defense in nearly all cells exposed to oxygen. There are three major families of superoxide dismutase, depending on the metal cofactor: Cu-Zn (which binds both copper and zinc), Fe and Mn types (which bind either iron or manganese), and finally the Ni type, which binds nickel.Mouse anti Bovine superoxide dismutase (Cu/Zn) antibody, clone 2F5 does not cross react with immunoglobulins but does react weakly with Mn SOD.
产品详情
Target SpeciesBovine
Product FormPurified IgG - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
Fusion PartnersCells from immunised mice were fused with cells of the mouse Sp-2/0-Ag14 myeloma cell line.
ImmunogenSuperoxide dismutase enzyme prepared from bovine erythrocytes.
Preservative Stabilisers0.1%Sodium Azide (NaN3)0.1%Bovine Serum Albumin
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
文献
1.Paździor-Czapula, K.et al.(2014) Immunohistochemical evaluation of superoxide dismutase (Cu/Zn SOD) concentrations in erythrocytes of dairy cattle and farm-raised deer by a computer-assisted analysis of microscopic images.Pol J Vet Sci. 17: 275-9.

技术参数

产品应用 IHC(F);ELISA;IHC(P)

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