Bio-Rad MCA2459A647 CD138,Mouse anti Human CD138:Alexa Fluor647

2024-10-22

Mouse anti Human CD138:Alexa Fluor647

货号:MCA2459A647,MCA2459A647T

规格:100 Tests/1ml,25 Tests/0.25ml

价格:3995,1445

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD138

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号:B-A38

荧光染料:Alexa Fluor 647

产品介绍
Mouse anti human CD138 antibody, clone B-A38recognizes human CD138, also known as Syndecan-1 (SDC-1). CD138 is a member of the transmembrane heparan sulfate proteoglycan family (O’Connellet al.2004,Sandersonet al.2008). It is composed of a core protein (comprising 3 domains; a short cytoplasmic domain, a transmembrane domain, and a long extracellular domain) and covalently attached heparan sulfate chains (Sandersonet al.2008).Syndecan-1 is expressed on the surface of plasma cells within the hematopoietic system and on the surface of mature epithelial cells (O’Connellet al.2004). It acts as an extracellular matrix receptor, involved in many cellular functions, including cell binding, cell signaling and cytoskeletal organization through cell-cell adhesion and cell-matrix adhesion (Sandersonet al.2008).
产品详情
Target SpeciesHuman
Product FormPurified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation
  • MCA2459A647: Purified IgG prepared by ion exchange chromatography.
  • MCA2459A647T: Purified IgG prepared by ion exchange chromatography
Fusion PartnersSpleen cells from immunized Balb/c (Iffa Credo) mice were fused with cells of the mouse X63/Ag.8653 myeloma cell line.
Buffer SolutionPhosphate buffered saline
ImmunogenU266 cell line.
Approx. Protein ConcentrationsIgG concentration 0.1 mg/ml
Preservative Stabilisers0.09%Sodium Azide1%Bovine Serum Albumin
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
Alexa Fluor®647650665
AcknowledgementsThis product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
推荐产品
DescriptionProduct CodeApplicationsPack Size
Mouse IgG1 Negative Control:Alexa Fluor® 647MCA928A647F100 Tests/1ml
文献
1.Elbezanti, O.W.et al.(2022) Development of a Novel Bruton’s Tyrosine Kinase Inhibitor that exerts Anti-Cancer Activities Potentiates Response of Chemotherapeutic Agents In Multiple Myeloma Stem Cell-Like CellsbioRxiv 2022.03.10.483708 [Epub ahead of print].2.Borset, M.et al.(1993) Lack of IL-1 secretion from human myeloma cells highly purified by immunomagnetic separation.Br J Haematol. 85 (3): 446-51.3.Du, S.et al.(2010) Systemic mastocytosis in association with chronic lymphocytic leukemia and plasma cell myeloma.Int J Clin Exp Pathol. 3 (4): 448-57.4.Kylänpää, L.et al.(2009) Syndecan-1 and tenascin expression in cystic tumors of the pancreas.JOP. 10 (4): 378-82.

技术参数

产品应用 MCA2459A647/MCA2459A647T:Flow Cytometry

产品类型 Monoclonal Antibody

推荐用于 MCA2459A647/MCA2459A647T:Flow Cytometry

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