Bio-Rad m bovine CD335:Alexa Fluor 488 MCA2365A488,mouse anti bovine CD335:Alexa Fluor 488

2024-10-22

mouse anti bovine CD335:Alexa Fluor 488

货号:MCA2365A488

规格:100 Tests/1ml

价格:4165

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD335

物种:Bovine

宿主:小鼠

抗体亚型:IgG1

克隆号:AKS1

荧光染料:Alexa Fluor 488

产品介绍
Mouse anti Bovine CD335 antibody, clone AKS1recognizes bovine CD335, also known as NKp46 and Natural cytotoxicity triggering receptor 1. CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which functions as an activating receptor. CD335 is expressed by human natural killer cells (Sivoriet al.1997). The bovine homologue is expressed on bovine NK cells (Storsetet al.2004) and no expression of CD335 has been detected on B cells, T cells, monocytes or granulocytes.Clone AKS1 is reported to activate lysis of FcgammaR-expressing cell line P815, by IL-2 activated NKp46+ cells (Storsetet al.2004).
产品详情
Target SpeciesBovine
Product FormPurified IgG conjugated to Alexa Fluor® 488 - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
AcknowledgementsThis product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09%Sodium Azide1%Bovine Serum Albumin
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
Alexa Fluor®488495519
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Storset, A.K.et al.(2004) NKp46 defines a subset of bovine leukocytes with natural killer cell characteristics.Eur J Immunol. 34 (3): 669-76.2.Kulberg, S.et al.(2004) Reference values for relative numbers of natural killer cells in cattle blood.Dev Comp Immunol. 28 (9): 941-8.3.Van Rhijn, I.et al.(2007) Massive, sustained γ/δ T cell migration from the bovine skinin vivo.J Leukoc Biol. 81: 968-73.4.Toka, F.N.et al.(2011) Rapid and Transient Activation of γ/δ T Cells to IFN-γ Production, NK Cell-Like Killing, and Antigen Processing during Acute Virus Infection.J Immunol. 186: 4853-61.

技术参数

产品应用 Flow

产品类型 单克隆抗体

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