BIO-RAD MCA2250 Rat anti Human Interleukin-10 antibody, clone JES3-12G8,Rat anti Human Interleukin-10 antibody, clone JES3-1

2024-10-22

Rat anti Human Interleukin-10 antibody, clone JES3-12G8

货号:MCA2250

规格:0.5 mg

价格:3936

产品类型:流式抗体

品牌:Bio-Rad

抗原:Interleukin-10

物种:人

宿主:大鼠

抗体亚型:IgG2a

克隆号:JES3-12G8

荧光染料:Purified

产品介绍
Rat anti Human Interleukin-10 antibody, clone JES3-12G8recognizes human Interleukin-10 (IL-10). IL-10 is expressed by activated CD8+ peripheral blood T-cells, activated T-helper CD4+ T cell clones mast cells, B cell lymphomas and LPS activated monocytes.Rat anti Human Interleukin-10 antibody, clone JES3-12G8 is reported to neutralize the effects of IL-10 (Abramset al.1992).
产品详情
Target SpeciesHuman
Product FormPurified IgG - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Fusion PartnersSpleen cells from immunized Balb/c were fused with cells of the SP2/0-Ag14 mouse myeloma cell line
Buffer SolutionPhosphate buffered saline
ImmunogenHuman IL-10 expressed by COS cells.
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
Preservative Stabilisers0.01%Sodium Azide
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Burdin, N.et al.(1993) Epstein-Barr virus transformation induces B lymphocytes to produce human interleukin 10.J Exp Med. 177 (2): 295-304.2.Yssel, H.et al.(1992) IL-10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells.J Immunol. 149 (7): 2378-84.3.Abrams, J.S.et al.(1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.Immunol Rev. 127: 5-24.4.O'Farrell, A.M.et al.(1998) IL-10 inhibits macrophage activation and proliferation by distinct signaling mechanisms: evidence for Stat3-dependent and -independent pathways.EMBO J. 17 (4): 1006-18.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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