BIO-RAD MCA2061FT Mouse anti Human CD284 antibody, clone HTA125,Mouse anti Human CD284 antibody, clone HTA125-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

BIO-RAD MCA2061FT Mouse anti Human CD284 antibody, clone HTA125,Mouse anti Human CD284 antibody, clone HTA125

2024-10-22

Mouse anti Human CD284 antibody, clone HTA125

货号：MCA2061FT,MCA2061F

规格：0.1 mg,0.2 mg

价格：893,3162

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD284

物种：人

宿主：小鼠

抗体亚型：IgG2a

克隆号：HTA125

荧光染料：FITC

产品介绍
Mouse anti Human CD284 antibody, clone HTA125recognizes the human Toll like receptor 4 (TLR4) cell surface antigen.TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule.TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.This antibody has been demonstrated to block activation of monocytes with LPS. The use of a preservative free format of Mouse anti Human CD284 antibody, clone HTA125 (MCA2061EL) is recommended for functional assays.
产品详情
Target Species	Human
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	MCA2061F: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant MCA2061FT: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09%Sodium Azide
Immunogen	Ba/F3 cell line expressing TLR4 (CD284).
Fusion Partners	Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Approx. Protein Concentrations	IgG concentration 0.1mg/ml
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Foster, N.et al.(2007) Pivotal advance: vasoactive intestinal peptide inhibits up-regulation of human monocyte TLR2 and TLR4 by LPS and differentiation of monocytes to macrophages.J Leukoc Biol. 81: 893-903.2.Shimazu, R.et al.(1999) MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4.J Exp Med. 189 (11): 1777-82.3.Jiang, Q.et al.(2000) Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-kappa B.J Immunol. 165 (7): 3541-4.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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BIO-RAD MCA2061FT Mouse anti Human CD284 antibody, clone HTA125,Mouse anti Human CD284 antibody, clone HTA125

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