BIO-RAD MCA2050 Mouse anti Huntingtin antibody, clone HDB4E10,Mouse anti Huntingtin antibody, clone HDB4E10

2024-10-22

Mouse anti Huntingtin antibody, clone HDB4E10

货号:MCA2050

规格:0.1 mg

价格:2576

产品类型:流式抗体

品牌:Bio-Rad

抗原:Huntingtin

物种:人/小鼠

宿主:小鼠

抗体亚型:IgG1

克隆号:HDB4E10

荧光染料:Purified

产品介绍
Mouse anti Huntingtin antibody, clone HDB4E10reacts with an epitope corresponding to the HDB region (amino acids 1844 - 2131) of the huntingtin protein.Mouse anti Huntingtin antibody, clone HDB4E10 detects a ~350 kDa band on western blots but also detects smaller degradation products of huntingtin. The combined use of Mouse anti Huntingtin antibody, clones HDB4E10 (MCA2050) and HDC8A4 (MCA2051) demonstrate that huntingtin is enriched in neuronal cells in the brain (Jones 1999).
产品详情
Target SpeciesHuman
Product FormPurified IgG - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
ImmunogenRecombinant protein corresponding to amino acids 1844 - 2131 of huntingtin.
Preservative Stabilisers<0.1% Sodium Azide (NaN3)
Approx. Protein ConcentrationsIgG concentration 1.0mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry1/25
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Wilkinson, F. L.et al. (1999) Localization of rabbit huntingtin using a new panel of monoclonal antibodies.Molecular Brain Research. 69: 10-20.2.Yao Yet al.(2015) A striatal-enriched intronic GPCR modulates huntingtin levels and toxicity.Elife. 4: 4.3.Shirasaki, D.I.et al.(2012) Network organization of the huntingtin proteomic interactome in mammalian brain.Neuron. 75 (1): 41-57.

技术参数

产品应用 WB

产品类型 Monoclonal Antibody

推荐用于 WB

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