BIO-RAD MCA2028A647 Mouse anti Human CD29 monoclonal antibody, clone 12G10 ,Mouse anti Human CD29 monoclonal antibody, clon

2024-10-22

Mouse anti Human CD29 monoclonal antibody, clone 12G10

货号:MCA2028A647

规格:100 Tests/1ml

价格:3188

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD29

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号:12G10

荧光染料:Alexa Fluor 647

产品介绍
Mouse anti Human CD29 monoclonal antibody, clone 12G10recognizes human CD29 also known as beta1 integrin or VLA-4 subunit alpha. CD29 is a ~130 kDa under reducing, ~115 kDa under non-reducing conditions single pass type I transmembrane glycoprotein. CD29 acts as the common beta subunit of the heterodimeric very late antigens 1-6, complexing with CD49a-f respectively where it forms part of the receptors for laminin , collagen and fibronectin. the VLA heterodimers mediate cell-cell and cell-matrix interactions.Mouse anti Human CD29, clone 12G10 binding to cells adhering via VLA-4 results in actin cytoskeletal disruption and subsequent inhibition of attachment and spreading whilst 12G10 binding to cells adhering via VLA-5 results in enhancement of both these processes (Humphrieset al.2005). Clone 12G10 enhances alpha 5 beta 1-fibronectin interactions and binds to a region of CD25 containing the binding epitopes of several other anti CD29 antibody clones. However, unlike these, binding of 12G10 is enhanced in the presence of ligands such as fibronectin fragments (Mouldet al.1995). Binding of antibody clone 12G10 to the integrin β1 subunit is affected by divalent cations and the binding epitope appears to be located around residues 207-218 in the b1 subunit putative A-domain (Mouldet al.1998).
产品详情
Target SpeciesHuman
Product FormPurified IgG conjugated to Alexa Fluor 647 - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
ImmunogenPurified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Approx. Protein ConcentrationsIgG concentration 0.05 mg/ml
Fusion PartnersSpleen cells from an immunised BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
Alexa Fluor®647650665
AcknowledgementsThis product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
推荐产品
DescriptionProduct CodeApplicationsPack Size
Rat IgG2a Negative Control:Alexa Fluor® 647MCA1212A647F100 Tests/1ml
文献
1.Sodek, K.L.et al.(2009) Compact spheroid formation by ovarian cancer cells is associated with contractile behavior and an invasive phenotype.Int J Cancer. 124: 2060-70.2.Mould, A.P.et al.(1995) Regulation of integrin alpha 5 beta 1 function by anti-integrin antibodies and divalent cations.Biochem Soc Trans. 23 (3): 395S.3.Mould, A.P.et al.(1995) Identification of a novel anti-integrin monoclonal antibody that recognises a ligand-induced binding site epitope on the beta 1 subunit.FEBS Lett. 363 (1-2): 118-22.4.Matthews,B.D.et al.(2010) Ultra-rapid activation of TRPV4 ion channels by mechanical forces applied to cell surface [beta]1 integrins.Integr Biol (Camb). 2: 435-42.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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