IL-2 Human ELISA Kit,IL-2 Human ELISA Kit/人IL-2 ELISA试剂盒

2024-10-23

IL-2 Human ELISA Kit/人IL-2 ELISA试剂盒

货号:BMS221-2,BMS221-2TWO,BMS221-2FIVE,BMS221-2TEN

规格:96 Tests,2X96 Tests,5X96 Tests,10X96 Tests

价格:1,1,18524,34832

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

9.1 pg/mL

检测范围18.8-1,200 pg/mL

样本类型/体积

血清 50 μL

血浆 50 μL

上清液 50 μL

操作时间

1 hr 20 min

测试时间

3 hr 30 min

Homogenous (no wash)

No

批间变异系数(CV)5%
批内变异系数(CV)7%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 对照

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameIL-2
Protein aliasesAldesleukin, IL-2, T-cell growth factor, TCGF, Interleukin-2, involved in regulation of T-cell clonal expansion, T cell growth factor

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesIL-2, IL2, lymphokine, TCGF
Gene ID(Human) 3558
Gene symbolIL2
UniProt ID(Human) P60568

The Human Interleukin-2 (Hu IL-2) ELISA quantitates Hu IL-2 in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-2.人白介素2(人IL-2)ELISA定量了人血清,血浆或细胞培养基中的人IL-2。该检测试剂盒将专门识别天然和重组人IL-2。Principle of the method 方法原理The Human IL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-2固相夹心ELISA(酶联免疫吸附测定)旨在测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.白介素2(IL-2)是一种免疫调节性细胞因子,对于活化T细胞的增殖,B细胞,自然杀伤细胞,单核细胞和巨噬细胞的分化非常重要。 IL-2通过IL-2受体(IL-2R)发出信号,IL-2R是一种异源三聚体蛋白复合物,其γ链也被白介素4(IL-4)和白介素7(IL-7)共享。 IL-2基因在成熟胸腺细胞中的表达是单等位基因,代表了控制单个基因精确表达的异常调节模式。 在小鼠中类似于IL-2的基因的定向破坏会导致溃疡性结肠炎样疾病,这表明该基因在对抗原刺激的免疫反应中起着至关重要的作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

IL-2 Human ELISA KitRepresentative standard curve for Human IL-2 ELISA

技术参数
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