Bio-Rad MCA1940SBV670 CD19抗体,mouse anti human CD19:StarBright Violet 670-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1940SBV670 CD19抗体,mouse anti human CD19:StarBright Violet 670

2024-10-22

mouse anti human CD19:StarBright Violet 670

货号：MCA1940SBV670

规格：100 Tests/0.5ml

价格：5466

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD19

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：LT19

荧光染料：其它

产品介绍
Mouse anti Human CD19 antibody, clone LT19recognizes human CD19 also known as T-cell surface antigen Leu-12 or B-lymphocyte surface antigen B4. CD19 is a ~95 kDa type I single pass transmembrane glycoprotein expressed on follicular dendritic cells and B-cells during maturation but is lost on development into plasma cells (de Rieet al.1989).CD19 is the broadest lineage specific marker for B cells and functions as a B-cell co-receptor in conjunction with CD21 (Bradburyet al.1992), CD9, CD81 and CD82 (Horváthet al.1998). CD19 is implicated in the down-regulation of B cell growth and proliferation (Pezzuttoet al.1987).
产品详情
Target Species	Human
Product Form	Purified IgG conjugated to StarBright Violet 670- liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin0.1% Pluronic F680.1% PEG 3350
Immunogen	Normal human peripheral blood lymphocytes.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
StarBright Violet 670	400	667
Regulatory	For research purposes only
Guarantee	12 monthsfrom dateof despatch
Acknowledgements	This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
存储条件
Store at +4°C. DO NOT FREEZE.This product should be stored undiluted.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat	1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude itsuse in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 5ul of the suggested working dilution to label 106cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
数据
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD20 (MCA1710A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD20 (MCA1710A488) and StarBright Violet 670 conjugated Mouse anti Human CD19 (MCA1940SBV670). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
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文献
1. Girbl, T.et al.(2013) CD40-mediated activation of chronic lymphocytic leukemia cells promotes their CD44-dependent adhesion to hyaluronan and restricts CCL21-induced motility.Cancer Res. 73: 561-70.2. Hertzberg, L.et al.(2010) Down syndrome acute lymphoblastic leukemia, a highly heterogeneous disease in which aberrant expression of CRLF2 is associated with mutated JAK2: a report from the International BFM Study Group.Blood. 115: 1006-17.3. Kakko, T.et al.(2011) Inflammatory effects of blood leukocytes: association with vascular function in neuropeptide Y proline 7-genotyped type 2 diabetes patients.Diab Vasc Dis Res. 8: 221-8.4. Dorvignit, D.et al.(2012) Expression and biological characterization of an anti-CD20 biosimilar candidate antibody: a case study.MAbs. 4 (4): 488-96.5. Karlsen, M.et al.(2015) TLR-7 and -9 Stimulation of Peripheral Blood B Cells Indicate Altered TLR Signalling in Primary Sjögren's Syndrome Patients by Increased Secretion of Cytokines.Scand J Immunol. 82 (6): 523-31.6. Clark, L.E.et al.(2018) Vaccine-elicited receptor-binding site antibodies neutralize two New World hemorrhagic fever arenaviruses.Nat Commun. 9 (1): 1884.7. Gu, Y.et al.(2019) Defining the structural basis for human alloantibody binding to human leukocyte antigen allele HLA-A*11:01.Nat Commun. 10 (1): 893.8. Yang, C.et al.(2013) B cells promote tumor progression via STAT3 regulated-angiogenesis.PLoS One. 8 (5): e64159.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Bio-Rad MCA1940SBV670 CD19抗体,mouse anti human CD19:StarBright Violet 670

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