产品介绍 | | |
Rat anti Mouse CD68 antibody, clone FA-11recognizes mouse macrosialin, a heavily glycosylated transmembrane protein and murine homolog of human CD68, classified as a unique scavenger receptor (ScR) family member, due to the presence of a lysosome associated membrane protein (LAMP)-like domain.CD68 is considered a pan macrophage marker, predominantly expressed on the intracellular lysosomes of tissue macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes.CD68 is expressed by many tumor types including some B cell lymphomas, blastic NK lymphomas, melanomas, granulocytic (myeloid) sarcomas, hairy cell leukemias, and renal, urinary and pancreatic tumors, and can be used to demonstrate the presence/localization of macrophages.Rat anti mouse CD68 antibody, clone FA-11, has been used in many mouse models for the identification of CD68 in immunohistochemical assays, using both frozen and paraffin-embedded tissues (Masakiet al.2003) and (Deveyet al.2009).Rat anti mouse CD68 antibody, clone FA-11 can be used in flow cytometry to detect intracellular CD68, following permeabilization, and can detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation. |
产品详情 | | |
Target Species | Mouse |
Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
Reconstitution | Pack Size: 100 Tests Reconstitute with 1.0 ml distilled water Pack Size: 25 Tests Reconstitute in 0.25 ml disilled water |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09%Sodium Azide 1% Bovine Serum Albumin 5% Sucrose |
Immunogen | Purified Concanavalin A acceptor glycoprotein from P815 cell line. |
Approx. Protein Concentrations | IgG concentration 0.1 mg/ml |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
RPE 488nm laser | 496 | 578 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom dateof despatch |
存储条件 | | |
Pack Size: 100 TestsStore at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 25 TestsStore at +4°C.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry1 | √ | | Neat |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Membrane permeabilization is required for this application. The use of Leucoperm (Product CodeBUF09) is recommended for this purpose..The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (Product CodeBUF041).Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
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同型 |
Description | Product Code | Applications | Pack Size |
Rat IgG2a Negative Control:RPE | MCA1212PE | Flow | 100 Tests |
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文献 |
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