| 产品介绍 | | |
| Rat anti Mouse CD68 antibody, clone FA-11recognizes mouse macrosialin, a heavily glycosylated transmembrane protein and murine homolog of human CD68, classified as a unique scavenger receptor (ScR) family member, due to the presence of a lysosome associated membrane protein (LAMP)-like domain.CD68 is considered a pan macrophage marker, predominantly expressed on the intracellular lysosomes of tissue macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes.CD68 is expressed by many tumor types including some B cell lymphomas, blastic NK lymphomas, melanomas, granulocytic (myeloid) sarcomas, hairy cell leukemias, and renal, urinary and pancreatic tumors, and can be used to demonstrate the presence/localization of macrophages.Rat anti mouse CD68 antibody, clone FA-11, has been used in many mouse models for the identification of CD68 in immunohistochemical assays, using both frozen and paraffin-embedded tissues (Masakiet al.2003) and (Deveyet al.2009).Rat anti mouse CD68 antibody, clone FA-11 can be used in flow cytometry to detect intracellular CD68, following permeabilization, and can detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation. |
| 产品详情 | | |
Target Species | Mouse |
Product Form | Purified IgG - liquid |
Preparation | MCA1957, MCA1957GA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant MCA1957T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
|
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09%Sodium Azide |
| Carrier Free | Yes |
Immunogen | Purified Concanavalin A acceptor glycoprotein from P815 cell line. |
| Approx. Protein Concentrations | Pack Size: 0.25 mg, 25 µg IgG concentration 1.0mg/ml Pack Size: 0.1 mg IgG concentration 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom dateof despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry1 | √ | 1/50 | 1/100 |
| Immunofluorescence | √ | | |
| Immunohistology - Frozen | √ | | |
| Immunohistology - Paraffin2 | √ | | |
| Immunoprecipitation | √ | | |
| Western Blotting3 | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product CodeBUF09) for this purpose.2This product may require antigen retrieval using heat treatment prior to staining of paraffin sections. Either sodium citrate buffer or Tris/EDTA buffer may be used for this purpose (Martin-Mansoet al. 2008). Staining has also been achieved without antigen retrieval (Luet al. 2010).3Non-reducing conditions recommended.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
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| 同型 |
| Description | Product Code | Applications | Pack Size |
| Rat IgG2a Negative Control | MCA1212 | Flow | 1 mL |
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| 文献 |
| 1. Bardin, M.et al.(2022) The resolvin D2 - GPR18 axis is expressed in human coronary atherosclerosis and transduces atheroprotection in apolipoprotein E deficient mice.Biochem Pharmacol. : 115075.2. Miller-Rhodes, P.et al.(2022) URMC-099 prophylaxis prevents hippocampal vascular vulnerability and synaptic damage in an orthopedic model of delirium superimposed on dementia.FASEB J. 36 (6): e22343.3. Kovács, A.D.et al.(2022) Acidified drinking water attenuates motor deficits and brain pathology in a mouse model of a childhood neurodegenerative disorder.Sci Rep. 12 (1): 9025.4. Agrawal, S.et al.(2022) Impact of IL-21-associated peripheral and brain crosstalk on the Alzheimer's disease neuropathology.Cell Mol Life Sci. 79 (6): 331.5. Piec, P.A.et al.(2022) Muramyl Dipeptide Administration Delays Alzheimer's Disease Physiopathology via NOD2 Receptors.Cells. 11 (14): 2241.6. Yamasaki, T.et al.(2022) Inhibition of microRNA-33b specifically ameliorates abdominal aortic aneurysm formation via suppression of inflammatory pathways.Sci Rep. 12 (1): 11984.7. Burger, F.et al.(2022) The E3 Ubiquitin Ligase Peli1 Deficiency Promotes Atherosclerosis Progression.Cells. 11 (13): 2014.8. Lam, S.et al.(2022) Pathological changes induced by Alzheimer's brain inoculation in amyloid-beta plaque-bearing mice.Acta Neuropathol Commun. 10 (1): 112.9. Uchida, H.A.et al.(2022) Edaravone Attenuated Angiotensin II-Induced Atherosclerosis and Abdominal Aortic Aneurysms in Apolipoprotein E-Deficient Mice.Biomolecules. 12(8):1117. |
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