Bio-Rad MCA1957A488T CD68抗体,Rat anti mouse CD68:Alexa Fluor® 488

2024-10-22

Rat anti mouse CD68:Alexa Fluor® 488

货号:MCA1957A488T ,MCA1957A488

规格:25 Tests/0.25ml,100 Tests/1ml

价格:1233,3315

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD68

物种:小鼠

宿主:大鼠

抗体亚型:IgG2a, kappa

克隆号:FA-11

荧光染料:Alexa Fluor 488

产品介绍
Rat anti Mouse CD68 antibody, clone FA-11recognizes mouse macrosialin, a heavily glycosylated transmembrane protein and murine homolog of human CD68, classified as a unique scavenger receptor (ScR) family member, due to the presence of a lysosome associated membrane protein (LAMP)-like domain.CD68 is considered a pan macrophage marker, predominantly expressed on the intracellular lysosomes of tissue macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes.CD68 is expressed by many tumor types including some B cell lymphomas, blastic NK lymphomas, melanomas, granulocytic (myeloid) sarcomas, hairy cell leukemias, and renal, urinary and pancreatic tumors, and can be used to demonstrate the presence/localization of macrophages.Rat anti mouse CD68 antibody, clone FA-11, has been used in many mouse models for the identification of CD68 in immunohistochemical assays, using both frozen and paraffin-embedded tissues (Masakiet al.2003) and (Deveyet al.2009).Rat anti mouse CD68 antibody, clone FA-11 can be used in flow cytometry to detect intracellular CD68, following permeabilization, and can detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
产品详情

Target Species

Mouse

Product Form

Purified IgG conjugated to Alexa Fluor® 488 - liquid

Preparation

  • MCA1957A488: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

  • MCA1957A488T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09%Sodium Azide

1%Bovine Serum Albumin

Immunogen

Purified Concanavalin A acceptor glycoprotein from P815 cell line.

Approx. Protein ConcentrationsIgG concentration 0.05 mg/ml
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
Alexa Fluor®488495519

Regulatory

For research purposes only

Guarantee

12 monthsfrom dateof despatch

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution

Flow Cytometry

Neat1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude itsuse in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
同型
DescriptionProduct CodeApplicationsPack Size
Rat IgG2a Negative Control:Alexa Fluor® 488MCA1212A488

Flow

100 Tests/1ml
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文献
1. Bardin, M.et al.(2022) The resolvin D2 - GPR18 axis is expressed in human coronary atherosclerosis and transduces atheroprotection in apolipoprotein E deficient mice.Biochem Pharmacol. : 115075.2. Miller-Rhodes, P.et al.(2022) URMC-099 prophylaxis prevents hippocampal vascular vulnerability and synaptic damage in an orthopedic model of delirium superimposed on dementia.FASEB J. 36 (6): e22343.3. Kovács, A.D.et al.(2022) Acidified drinking water attenuates motor deficits and brain pathology in a mouse model of a childhood neurodegenerative disorder.Sci Rep. 12 (1): 9025.4. Agrawal, S.et al.(2022) Impact of IL-21-associated peripheral and brain crosstalk on the Alzheimer's disease neuropathology.Cell Mol Life Sci. 79 (6): 331.5. Piec, P.A.et al.(2022) Muramyl Dipeptide Administration Delays Alzheimer's Disease Physiopathology via NOD2 Receptors.Cells. 11 (14): 2241.6. Yamasaki, T.et al.(2022) Inhibition of microRNA-33b specifically ameliorates abdominal aortic aneurysm formation via suppression of inflammatory pathways.Sci Rep. 12 (1): 11984.7. Burger, F.et al.(2022) The E3 Ubiquitin Ligase Peli1 Deficiency Promotes Atherosclerosis Progression.Cells. 11 (13): 2014.8. Lam, S.et al.(2022) Pathological changes induced by Alzheimer's brain inoculation in amyloid-beta plaque-bearing mice.Acta Neuropathol Commun. 10 (1): 112.9. Uchida, H.A.et al.(2022) Edaravone Attenuated Angiotensin II-Induced Atherosclerosis and Abdominal Aortic Aneurysms in Apolipoprotein E-Deficient Mice.Biomolecules. 12(8):1117.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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