Bio-Rad MCA1878F CD97抗体,mouse anti human CD97:FITC

2024-10-22

mouse anti human CD97:FITC

货号:MCA1878F

规格:0.1 mg

价格:2576

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD97

物种:人

宿主:小鼠

抗体亚型:IgG1, kappa

克隆号:MEM-180

荧光染料:FITC

产品介绍
Mouse anti Human CD97 antibody, clone MEM-180recognizes the human CD97 cell surface antigen, a 74-89 kDa glycoprotein expressed by granulocytes, monocytes, some activated lymphocytes and weakly by resting lymphocytes (5-15%). Mouse anti Human CD97 antibody, clone MEM-180 has been reported to detect CD97 antigen weakly in western blotting.
产品详情

Target Species

Human

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from ascites

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09%Sodium Azide

1%Bovine Serum Albumin

Approx. Protein Concentrations

IgG concentration 0.1mg/ml

Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
FITC490525
Fusion PartnersSpleen cells from immunized Balb/c mice were fused with SP2/0 mouse myeloma cells.

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul whole blood
同型
DescriptionProduct CodeApplicationsPack Size
Mouse IgG1 Negative Control:FITCMCA928FFlow100 Tests
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文献
1. Steinert, M.et al.(2002) Expression and regulation of CD97 in colorectal carcinoma cell lines and tumor tissues.Am J Pathol. 161 (5): 1657-67.2. Hamann, J.et al.(1998) Characterization of the CD55 (DAF)-binding site on the seven-span transmembrane receptor CD97.Eur J Immunol. 28 (5): 1701-7.3. Wobus, M.et al.(2004) N-glycosylation of CD97 within the EGF domains is crucial for epitope accessibility in normal and malignant cells as well as CD55 ligand binding.Int J Cancer. 112 (5): 815-22.4. Yona, S.et al.(2008) Ligation of the adhesion-GPCR EMR2 regulates human neutrophil function.FASEB J. 22 (3): 741-51.5. Wobus, M.et al.(2015) Association of the EGF-TM7 receptor CD97 expression with FLT3-ITD in acute myeloid leukemia.Oncotarget. 6 (36): 38804-15.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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