Bio-Rad MCA1870GT CD326抗体,mouse anti human CD326-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1870GT CD326抗体,mouse anti human CD326

2024-10-22

mouse anti human CD326

货号：MCA1870GT ,MCA1870G

规格：25 µg,0.2 mg

价格：893,3315

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD326

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：VU-1D9

荧光染料：Purified

产品介绍
Mouse anti Human CD326 antibody, clone VU-1D9recognizes epithelial cell adhesion molecule (Ep-CAM), a ~34 kDa cell surface antigen otherwise known as CD326, MOC31 or Ber-EP4. CD326 is a type 1 transmembrane glycoprotein, expressed on the basolateral cell membrane of the majority of epithelial tissues, with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells.Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells, andde novoexpression being seen in dysplastic squamous epithelium.
产品详情
Target Species	Human
Product Form	Purified IgG- liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)
Carrier Free	Yes
Immunogen	HG9 cell line (small cell lung carcinoma)
Approx. Protein Concentrations	1 mg/ml
Fusion Partners	Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	1/50	1/100
Immunohistology - Frozen	√
Immunohistology - Paraffin¹	√		1/200
Immunoprecipitation	√
Western Blotting	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100ul. Histology Positive Control TissueLarge Bowel Western BlottingMCA1870G detects a band of approximately 38kDa in HT-29 cell lysates
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control	MCA928	Flow	0.5 mg
文献
References for CD326 antibody1. Koga, Y.et al.(2008) Improved recovery of exfoliated colonocytes from feces using newly developed immunomagnetic beads.Gastroenterol Res Pract. 2008: 605273.2. Fillmore, C.M.et al.(2010) Estrogen expands breast cancer stem-like cells through paracrine FGF/Tbx3 signaling.Proc Natl Acad Sci U S A. 107 (50): 21737-42.3. Murakata, A.et al.(2011) Gene expression signature of the gross morphology in hepatocellular carcinoma.Ann Surg. 253: 94-100.4. Keller, P.J.et al.(2012) Defining the cellular precursors to human breast cancer.Proc Natl Acad Sci U S A. 109 (8): 2772-7.5. Jeong, H.T.et al.(2012) MRI features of hepatocellular carcinoma expressing progenitor cell markers.Liver Int. 32: 430-40.6. Armstrong, A.J.et al.(2019) Pharmacodynamic study of radium-223 in men with bone metastatic castration resistant prostate cancer.PLoS One. 14 (5): e0216934.Further Reading7. Winter, M.J.et al.(2003) The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology.Am J Pathol. 163 (6): 2139-48.

技术参数

产品应用 IHC(F); Flow; IP; IHC(P )* ;WB

产品类型 Monoclonal Antibody

推荐用于 IHC(F); Flow; IP; IHC(P )* ;WB

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Bio-Rad MCA1870GT CD326抗体,mouse anti human CD326

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