Bio-Rad MCA1853F CD163抗体,mouse anti human CD163:FITC-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1853F CD163抗体,mouse anti human CD163:FITC

2024-10-22

mouse anti human CD163:FITC

货号：MCA1853F

规格：3213

价格：3247

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD163

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：EDHu-1

荧光染料：FITC

产品介绍
Mouse anti Human CD163 antibody, clone EDHu-1recognizes the human CD163 cell surface antigen, a 130-140 kDa glycoprotein expressed by tissue macrophages. CD163 expression may be induced on monocytes by culture in dexamethasone.Clone EDHu-1 is reported to inhibit the binding of haptoglobin/hemoglobin to CD163 (Madsenet al.2004).Truncation mutation analysis demonstrates binding of EDHu-1 occurs via the N-terminal region of CD163 containing the first three scavenger receptor, Cysteine-rich,SRCR domainsthe third domain being critical as, cleavage of this domain at the major cleAavage siteASP-265abrogates binding to the N-terminal fragment.
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Rhesus Monkey	√
Sheep	√
Pig	√
Guinea Pig	√
Bovine	√
Cynomolgus monkey	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Immunogen	Leucocytes harvested from the pleural cavity of patients with idiopathic spontaneous pneumothorax
Approx. Protein Concentrations	IgG concentration 0.1 mg/ml
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from ligh
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat	1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 10ul of the suggested working dilution to label 10⁶cells in 100ul.
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control:FITC	MCA928F	Flow	100 Tests
相关产品
Description	Product Code	Applications	Pack Size
Mouse Seroblock FcR	BUF041A	Flow	0.1 mg
Mouse Seroblock FcR	BUF041B	Flow	0.5 mg
数据
Figure A. RPE conjugated mouse anti CD14 (MCA596PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated mouse anti CD14 (MCA596PE) and FITC conjugated mouse anti human CD163 (MCA1853F). All experiments performed on human peripheral blood mononuclear cells in the presence of Human SeroBlock (BUF070A).
文献
1. Frafjord, A.et al.(2020) Antibody combinations for optimized staining of macrophages in human lung tumours.Scand J Immunol. 92 (1): e12889.2. Ma, H.et al.(2021) Structural comparison of CD163 SRCR5 from different species sheds some light on its involvement in porcine reproductive and respiratory syndromevirus-2 infectionin vitro..Vet Res. 52 (1): 97.3. Kemmerer, C.L.et al.(2021) Cerebrospinal fluid cytokine levels are associated with macrophage infiltration into tumor tissues of glioma patientsBMC Cancer. 21(1):1108.4. Lehmann, M.et al.(2021) New onset of mainly guttate psoriasis after COVID-19 vaccination: a case report.J Eur Acad Dermatol Venereol. 35 (11): e752-e755.5. Baumann, D.et al.(2021) p38 MAPK signaling in M1 macrophages results in selective elimination of M2 macrophages by MEK inhibition.J Immunother Cancer.9 (7): e002319.6. Ikezumi, Y.et al.(2021) Steroid treatment promotes an M2 anti-inflammatory macrophage phenotype in childhood lupus nephritis.Pediatr Nephrol. 36 (2): 349-59.7. Eligini, S.et al.(2019) Biological profile of monocyte-derived macrophages in coronary heart disease patients: implications for plaque morphology.Sci Rep. 9 (1): 8680.8. Edwards, J.H.et al.(2021) Integration and functional performance of a decellularised porcine superflexor tendon graft in an ovine model of anterior cruciate ligament reconstruction.Biomaterials. 279: 121204.9. Ströbel, S.et al.(2021) A 3D primary human cell-based in vitro model of non-alcoholic steatohepatitis for efficacy testing of clinical drug candidates.Sci Rep. 11 (1): 22765.10. Galea, I.et al.(2022) Iron Deposition in the Brain After Aneurysmal Subarachnoid Hemorrhage.Stroke. 53 (5): 1633-42.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Bio-Rad MCA1853F CD163抗体,mouse anti human CD163:FITC

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