Bio-Rad MCA1846PE CD81抗体,Hamster anti mouse CD81: PE-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1846PE CD81抗体,Hamster anti mouse CD81: PE

2024-10-22

Hamster anti mouse CD81: PE

货号：MCA1846PE

规格：100 Tests

价格：3188

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD81

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG1, kappa

克隆号：Eat2

荧光染料：PE

产品介绍
Hamster anti Mouse CD81 antibody, clone Eat2recognizes mouse and rat CD81, also known as TAPA-1 or Target of the antiproliferative antibody 1. CD81 is a 236 amino acid ~26 kDa multipass transmembrane protein belonging to the TM4SF family (UniProt: P35762). In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. Hamster anti Mouse CD81 antibody, clone Eat2 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation (Maeckeret al.2000). Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding.Mice lacking CD81 demonstrate reduced fertility through impaired oocyte-sperm fusion, double knockout CD81-/- CD9-/- mice are completely infertile suggesting complimentary roles in oocyte-sperm fusion (Rubensteinet al.2006).
产品详情
Target Species	Mouse
Species Cross-Reactivity	Target Species	Cross Reactivity
Rat	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution	Reconstitute with 1.0 ml distilled water
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose
Immunogen	38C13, murine B cell line.
Approx. Protein Concentrations	IgG concentration 0.05 mg/ml
Fusion Partners	Spleen cells from immunised Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
RPE 488nm laser	496	578
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
Store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√		Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR
相关产品
Description	Product Code	Applications	Pack Size
Mouse Seroblock FcR	BUF041A	Flow	0.1 mg
Mouse Seroblock FcR	BUF041B	Flow	0.5 mg
文献
1. Conde-Vancells, J.et al.(2008) Characterization and comprehensive proteome profiling of exosomes secreted by hepatocytes.J Proteome Res. 7: 5157-66.2. Takeda, Y.et al.(2008) Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice.J Biol Chem. 283: 26089-97.3. Suzuki, M.et al.(2009) Tetraspanin CD9 negatively regulates lipopolysaccharide-induced macrophage activation and lung inflammation.J Immunol. 182: 6485-93.4. Ha, C.T.et al.(2005) Binding of pregnancy-specific glycoprotein 17 to CD9 on macrophages induces secretion of IL-10, IL-6, PGE2, and TGF-beta1.J Leukoc Biol. 77: 948-57.5. Pan, Q.et al.(2011) Hepatic cell-to-cell transmission of small silencing RNA can extend the therapeutic reach of RNA interference (RNAi).Gut. 61: 1330-9.6. Jin, Y.et al.(2013) Statins decrease lung inflammation in mice by upregulating tetraspanin CD9 in macrophages.PLoS One. 8: e73706.7. Royo, F.et al.(2013) Transcriptome of extracellular vesicles released by hepatocytes.PLoS One. 8: e68693.8. Owens, D.M. and Watt, F.M. (2001) Influence of beta1 integrins on epidermal squamous cell carcinoma formation in a transgenic mouse model: alpha3beta1, but not alpha2beta1, suppresses malignant conversion.Cancer Res. 61: 5248-54.9. Jin, Y.et al.(2018) Double deletion of tetraspanins CD9 and CD81 in mice leads to a syndrome resembling accelerated aging.Sci Rep. 8 (1): 5145.10. Sosa, L.J.et al.(2013) Amyloid precursor protein is an autonomous growth cone adhesion molecule engaged in contact guidance.PLoS One. 8 (5): e64521.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Bio-Rad MCA1846PE CD81抗体,Hamster anti mouse CD81: PE

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