Bio-Rad MCA1846T CD81抗体,Hamster anti mouse CD81

2024-10-22

Hamster anti mouse CD81

货号:MCA1846T ,MCA1846GA,MCA1846

规格:25 µg,0.1 mg,0.25 mg

价格:825,2023,3043

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD81

物种:小鼠

宿主:Armenian hamster仓鼠

抗体亚型:IgG1, kappa

克隆号:Eat2

荧光染料:Purified

产品介绍
Hamster anti Mouse CD81 antibody, clone Eat2recognizes mouse and rat CD81, also known as TAPA-1 or Target of the antiproliferative antibody 1. CD81 is a 236 amino acid ~26 kDa multipass transmembrane protein belonging to the TM4SF family (UniProt: P35762). In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. Hamster anti Mouse CD81 antibody, clone Eat2 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation (Maeckeret al.2000). Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding.Mice lacking CD81 demonstrate reduced fertility through impaired oocyte-sperm fusion, double knockout CD81-/- CD9-/- mice are completely infertile suggesting complimentary roles in oocyte-sperm fusion (Rubensteinet al.2006).
产品详情

Target Species

Mouse

Species Cross-ReactivityTarget SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide

Carrier FreeYes
Immunogen38C13, murine B cell line.
Approx. Protein Concentrations

Pack Size: 0.1 mg

IgG concentration 1 mg/ml

Pack Size: 25 µg, 0.25 mg

IgG concentration 1.0 mg/ml

Fusion PartnersSpleen cells from immunised Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line.

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution

ELISA

5 ug/ml as coating antibody
Flow Cytometry1/10Pack Size: 0.1 mg1/50Pack Size: 25 µg, 0.25 mg1/50Pack Size: 0.1 mg1/100Pack Size: 25 µg, 0.25 mg
Immunohistology - Frozen1
Immunohistology - Paraffinx
Immunoprecipitation
Western Blotting2
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.2Clone Eat2 recognizes mouse CD81 under non-reducing conditions.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
数据

Bovine GM-CSF ELISARecombinant Bovine GM-CSF detected using Mouse anti Bovine GM-CSF (MCA1828GA) as the capture reagent and a bovine reactive Mouse anti Sheep GM-CSF, clone 3C2 (MCA1924) conjugated to Biotin as the detection reagent followed by Streptavidin:HRP
文献
1. Conde-Vancells, J.et al.(2008) Characterization and comprehensive proteome profiling of exosomes secreted by hepatocytes.J Proteome Res. 7: 5157-66.2. Takeda, Y.et al.(2008) Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice.J Biol Chem. 283: 26089-97.3. Suzuki, M.et al.(2009) Tetraspanin CD9 negatively regulates lipopolysaccharide-induced macrophage activation and lung inflammation.J Immunol. 182: 6485-93.4. Ha, C.T.et al.(2005) Binding of pregnancy-specific glycoprotein 17 to CD9 on macrophages induces secretion of IL-10, IL-6, PGE2, and TGF-beta1.J Leukoc Biol. 77: 948-57.5. Pan, Q.et al.(2011) Hepatic cell-to-cell transmission of small silencing RNA can extend the therapeutic reach of RNA interference (RNAi).Gut. 61: 1330-9.6. Jin, Y.et al.(2013) Statins decrease lung inflammation in mice by upregulating tetraspanin CD9 in macrophages.PLoS One. 8: e73706.7. Royo, F.et al.(2013) Transcriptome of extracellular vesicles released by hepatocytes.PLoS One. 8: e68693.8. Owens, D.M. and Watt, F.M. (2001) Influence of beta1 integrins on epidermal squamous cell carcinoma formation in a transgenic mouse model: alpha3beta1, but not alpha2beta1, suppresses malignant conversion.Cancer Res. 61: 5248-54.9. Jin, Y.et al.(2018) Double deletion of tetraspanins CD9 and CD81 in mice leads to a syndrome resembling accelerated aging.Sci Rep. 8 (1): 5145.10. Sosa, L.J.et al.(2013) Amyloid precursor protein is an autonomous growth cone adhesion molecule engaged in contact guidance.PLoS One. 8 (5): e64521.

技术参数

产品应用 IHC(F) * ELISA; Flow;IP; WB *

产品类型 Monoclonal Antibody

推荐用于 IHC(F) * ELISA; Flow;IP; WB *

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