Bio-Rad MCA1825EL CD34抗体,Rat anti mouse CD34:Low Endotoxin

2024-10-22

Rat anti mouse CD34:Low Endotoxin

货号:MCA1825EL

规格:0.5 mg

价格:5100

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD34

物种:小鼠

宿主:大鼠

抗体亚型:IgG2a, kappa

克隆号:MEC14.7

荧光染料:其它

产品介绍
Rat anti Mouse CD34 antibody, clone MEC14.7recognizes the murine CD34 cell surface antigen, which is expressed by endothelial cells and by haematopoietic stem cells. This antibody recognizes a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Rat anti Mouse CD34 antibody, clone MEC14.7 appears to recognize a subset of the stem cell population recognized by clone RAM34, and it is thought that this is due to differences in the epitope recognized by the two antibodies.
产品详情

Target Species

Mouse

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

None present

Carrier FreeYes
ImmunogenT-end.1, a pMT transformed endothelial cell line.
Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Endotoxin Level<0.01 EU/ug

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
Store at -20oC only.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution

Flow Cytometry

Immunohistology - Paraffin1/201/200
Immunoprecipitation
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul
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文献
1. Winding, B.et al.(2002) Synthetic matrix metalloproteinase inhibitors inhibit growth of established breast cancer osteolytic lesions and prolong survival in mice.Clin Cancer Res. 8 (6): 1932-9.2. Nguyen, L.et al.(2012) Spatial morphological and molecular differences within solid tumors may contribute to the failure of vascular disruptive agent treatments.BMC Cancer. 12: 522.3. Morison, N.B.et al.(2007) The long-term actions of etonogestrel and levonorgestrel on decidualized and non-decidualized endometrium in a mouse model mimic some effects of progestogen-only contraceptives in women.Reproduction. 133: 309-21.4. Chen, L.et al.(2010) Roles of tetrahydrobiopterin in promoting tumor angiogenesis.Am J Pathol. 177: 2671-80.5. Ager, E.I.et al.(2010) Targeting the angiotensin II type 2 receptor (AT2R) in colorectal liver metastases.Cancer Cell Int. 10: 196. Chabot, S.et al.(2011) A novel antiangiogenic and vascular normalization therapy targeted against human CD160 receptor.J Exp Med. 208: 973-86.7. Chen, J.et al.(2011) Circulating endothelial progenitor cells and cellular membrane microparticles in db/db diabetic mouse: possible implications in cerebral ischemic damage.Am J Physiol Endocrinol Metab. 2011 Jul;301(1):E62-71.8. Chen, J.et al.(2012) Transfusion of CXCR4-primed endothelial progenitor cells reduces cerebral ischemic damage and promotes repair in db/db diabetic mice.PLoS One. 7 (11): e50105.9. Ouji, Y. & Yoshikawa, M. (2016) Maintenance of Skin Epithelial Stem Cells by Wnt-3a In Vitro.Methods Mol Biol. 1516: 279-88.10. Nguyen, L.et al.(2016) Vascular disruptive agent OXi4503 and anti-angiogenic agent Sunitinib combination treatment prolong survival of mice with CRC liver metastasis.BMC Cancer. 16 (1): 533.11. Vávrová, J.et al.(2012) Irradiated stem cells and ageing of the haematopoietic system.Radiat Environ Biophys. 51 (2): 205-13.12. DaCosta, P.L.N.et al.(2018) The kallikrein-Kinin system modulates the progression of colorectal liver metastases in a mouse model.BMC Cancer. 18 (1): 382.13. Danielyan, L.et al.. (2020) Cell motility and migration as determinants of stem cell efficacyEBioMedicine. 60:102989.14. Rackham, C.L.et al.(2013) Maintenance of islet morphology is beneficial for transplantation outcome in diabetic mice.PLoS One. 8 (2): e57844.15. Fruchon, S.et al.(2012) Involvement of the Syk-mTOR pathway in follicular lymphoma cell invasion and angiogenesis.Leukemia. 26 (4): 795-805.16. Zhu, C.et al.(2020) Antinociceptive effect of intrathecal injection of miR-9-5p modified mouse bone marrow mesenchymal stem cells on a mouse model of bone cancer pain.J Neuroinflammation. 17 (1): 85.

技术参数

产品应用 Flow; IP;IHC(P)

产品类型 Monoclonal Antibody

推荐用于 Flow; IP;IHC(P)

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