BIO-RAD MCA1899PE Mouse anti Horse Pan B-Cells:RPE,Mouse anti Horse Pan B-Cells:RPE

2024-10-22

Mouse anti Horse Pan B-Cells:RPE

货号:MCA1899PE

规格:100 Tests

价格:4352

产品类型:流式抗体

品牌:Bio-Rad

抗原:Pan B-Cells

物种:Horse

宿主:小鼠

抗体亚型:IgG1

克隆号:CVS36

荧光染料:PE

产品介绍
Rat anti Mouse CD115 antibody, clone 604B5 2E11recognizes the murine CD115 cell surface antigen, also known as the M-CSF receptor and as c-fms. Murine CD115 is a 958 amino acid 110kDa single pass type I transmembrane glycoprotein with tyrosine kinase activity and acts as a membrane receptor for M-CSF.CD115 is expressed by cells of the monocytic lineage and by progenitor cells. Rat anti Mouse CD115 antibody, clone 604B5 2E11 has been shown to inhibitin vitrocolony formation in response to M-CSF in both rats and mice.
产品详情
Target SpeciesMouse
Product FormPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
PreparationPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
ReconstitutionReconstitute with 1.0 ml distilled water
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin5% Sucrose
Fusion PartnersSpleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
ImmunogenPurified Equine Ig.
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
RPE 488nm laser496578
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Prior to reconstitution store at +4℃.After reconstitution store at +4℃.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
文献
1.Schneider, R. (2010) Analysis of antibody subtypes and T lymphocyte activation following vaccination of healthy foals against West Nile VirusIn M.Sc thesis University of Pennsylvania, Chapter 3, p.34-452.Lunn, D.P.et al.(1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.Vet Immunol Immunopathol. 62 (2): 101-43.3.Umlauf, C. (2004) Herstellung und Charakterisierung monoklonaler Antikörper gegen equine LeukozytenIn Phd thesis Ludwig-Maximillians-Universität München4.Tomlinson, J.E.et al.(2018) Multispectral fluorescence-activated cell sorting of B and T cell subpopulations from equine peripheral blood.Vet Immunol Immunopathol. 199: 22-31.5.Cequier, A.et al.(2022) Equine Mesenchymal Stem Cells Influence the Proliferative Response of Lymphocytes: Effect of Inflammation, Differentiation and MHC-Compatibility.Animals (Basel). 12 (8): 984.

技术参数

产品应用 Elisa;Flow

产品类型 Monoclonal Antibody

推荐用于 Elisa;Flow

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