BIO-RAD MCA1899GA Mouse anti Horse Pan B-Cells,Mouse anti Horse Pan B-Cells-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

BIO-RAD MCA1899GA Mouse anti Horse Pan B-Cells,Mouse anti Horse Pan B-Cells

2024-10-22

Mouse anti Horse Pan B-Cells

货号：MCA1899GA

规格：0.1 mg

价格：2720

产品类型：流式抗体

品牌：Bio-Rad

抗原：Pan B-Cells

物种：Horse

宿主：小鼠

抗体亚型：IgG1

克隆号：CVS36

荧光染料：Purified

产品介绍
Mouse anti Horse Pan B-Cells, clone CVS36is a monoclonal antibody directed against equine Ig light chains. Mouse anti Horse Pan B-Cells, clone CVS36 binds 100% of CD5-ve peripheral blood lymphocytes and recognized all equine B-cells (Lunnet al.1998) As the antigen recognized by clone CVS36 appears to be present on the surface of all equine B-cells it is therefore is a reagent that can be used as a pan B-cell marker for domestic horses (Breathnachet al.2005).Specific anti equine reagents have yet to be fully characterized for the typically recognized B-cell makers such as CD19, CD20, CD21, CD22 and CD79. While testing has demonstrated the cross reactivity of some monoclonal B-cell markers raised against other species with equine B cells, such as the Mouse anti Human CD79a antibody, (clone HM57), an overview of which may be found in the report of the second equine leucocyte antigen workshop (Lunnet al.1998).With specificity for equine Ig light chains, clone CVS36 may be used to detect all equine immunoglobulin classes and subclasses in ELISA applications (Lunnet al.1998).
产品详情
Target Species	Horse
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Approx. Protein Concentrations	IgG concentration 1.0mg/ml
Immunogen	Purified Equine Ig.
Fusion Partners	Spleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	1/20
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Schneider, R. (2010) Analysis of antibody subtypes and T lymphocyte activation following vaccination of healthy foals against West Nile VirusIn M.Sc thesis University of Pennsylvania, Chapter 3, p.34-452.Lunn, D.P.et al.(1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.Vet Immunol Immunopathol. 62 (2): 101-43.3.Umlauf, C. (2004) Herstellung und Charakterisierung monoklonaler Antikörper gegen equine LeukozytenIn Phd thesis Ludwig-Maximillians-Universität München4.Tomlinson, J.E.et al.(2018) Multispectral fluorescence-activated cell sorting of B and T cell subpopulations from equine peripheral blood.Vet Immunol Immunopathol. 199: 22-31.5.Cequier, A.et al.(2022) Equine Mesenchymal Stem Cells Influence the Proliferative Response of Lymphocytes: Effect of Inflammation, Differentiation and MHC-Compatibility.Animals (Basel). 12 (8): 984.

技术参数

产品应用 Elisa；Flow

产品类型 Monoclonal Antibody

推荐用于 Elisa；Flow

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BIO-RAD MCA1899GA Mouse anti Horse Pan B-Cells,Mouse anti Horse Pan B-Cells

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