BIO-RAD MCA1757PE Mouse anti Human Myeloperoxidase:RPE,Mouse anti Human Myeloperoxidase:RPE

2024-10-22

Mouse anti Human Myeloperoxidase:RPE

货号:MCA1757PE

规格:100 Tests

价格:3936

产品类型:流式抗体

品牌:Bio-Rad

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号:2C7

荧光染料:PE

产品介绍
Mouse anti Human myeloperoxidase antibody, clone 2C7recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55 kDa) and two light chains (15 kDa), the heavy chains being linked by a disulphide bond.Mouse anti Human Myeloperoxidase antibody, clone 2C7 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. Mouse anti Human Myeloperoxidase antibody, clone 2C7 also recognizes recombinant MPO in western blots and weakly in ELISA.Mouse anti Human myeloperoxidase antibody, clone 2C7 may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeabilization. Mouse anti Human myeloperoxidase antibody, clone 2C7 did not recognize rat MPO by ELISA (Patryet al.2003).
产品详情
Target SpeciesHuman
Product FormPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
PreparationPurified IgG prepared by affinity chromatography on Protein A
ReconstitutionReconstitute with 1 ml distilled water
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
ImmunogenHuman myeloperoxidase
Fusion PartnersSpleen cells from immunized mice were fused with cells of the mouse X63 AG8-653 myeloma cell line
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
RPE 488nm laser496578
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Prior to reconstitution store at +4℃. Following reconstitution store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Audrain, M.A.et al.(1997) Anti-native and recombinant myeloperoxidase monoclonals and human autoantibodies.Clin Exp Immunol. 107 (1): 127-34.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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