Rat anti Dog CD4 antibody, clone YKIX302.9, MCA1038A488 ,Rat anti Dog CD4 antibody, clone YKIX302.9,

2024-10-22

Rat anti Dog CD4 antibody, clone YKIX302.9,

货号:MCA1038A488

规格:100 Tests/1ml

价格:4208

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD4

物种:人

宿主:大鼠

抗体亚型:IgG2a

克隆号:YKIX302.9

荧光染料:Alexa Fluor 488

产品介绍
Rat anti Dog CD4 antibody, clone YKIX302.9, is a monoclonal antibody specific for the canine CD4 cell surface antigen. Clone YKIX302.9 was clustered at the first Canine Leukocyte Antigen Workshop (CLAW) [Cobboldet al.1992] along with cloneCA13.1E4.Rat anti Dog CD4 antibody, clone YKIX302.9 partially depletes circulating T lymphocytes when administeredin vivo, but alone is not sufficient to prolong allograft survival in a canine transplant model (Watsonet al.1993).Uniquely amongst mammals, canine CD4 is expressed by neutrophils as well as by lymphocyte subsets (Mooreet al.1992).
产品详情
Target SpeciesDog
Product FormPurified IgG conjugated to Alexa Fluor® 488 - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
ImmunogenConcanavilin A activated canine peripheral blood cells
Approx. Protein ConcentrationsIgG concentration 0.05mg/ml
Fusion PartnersSpleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
Alexa Fluor®488495519
AcknowledgementsThis product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood.
产品推荐
DescriptionProduct CodeApplicationsPack Size
Rat IgG2a Negative Control:Alexa Fluor® 488MCA1212A488F100 Tests/1ml
文献
1.Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens. 43 (3): 137-54.

技术参数

产品应用 Flow

产品类型 单克隆抗体

推荐用于 Flow

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