BIO-RAD MCA1749F Mouse anti Pig CD4 Alpha:FITC,Mouse anti Pig CD4 Alpha:FITC-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

BIO-RAD MCA1749F Mouse anti Pig CD4 Alpha:FITC,Mouse anti Pig CD4 Alpha:FITC

2024-10-22

Mouse anti Pig CD4 Alpha:FITC

货号：MCA1749F

规格：0.1 mg

价格：3740

产品类型：流式抗体

品牌：Bio-Rad

物种：pig

宿主：小鼠

抗体亚型：IgG2b

克隆号：MIL17

荧光染料：FITC

产品介绍
Mouse anti Porcine CD4 alpha, clone MIL17recognizes a ~55 kDa porcine homologue to the human CD4 antigen found on the surface of helper-T cells. MIL-17 was confirmed as a member of the CD4 alpha cluster at the ‘Third International Workshop on Swine Leukocyte Differentiation Antigens’ (Haversonet al.2001). Porcine CD4 is a type 1 trans-membrane member of the immunoglobulin superfamily.Pigs appear unusual amongst mammalian species as they appear to have four populations of resting T lymphocytes. In addition to the two populations of mutually exclusive CD4+/CD8- and CD4-/CD8+ lymphocytes, they also appear to have significant populations of CD4-/CD8- and CD4+/CD8+ cells. Lymphoblasts with a double positive phenotype have been described in other species but this is not the case for mature T lymphocytic calls (Saalmulleret al.1987)Mouse anti Pig CD4 alpha, clone MIL17 stains a population of cells with characteristic lymphocyte morphology in immunohistochemistry
产品详情
Target Species	Pig
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09%Sodium Azide1%Bovine Serum Albumin
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Immunogen	Leucocytes isolated from porcine gut lamina propria.
Approx. Protein Concentrations	IgG concentration 0.1mg/ml
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Saalmüller Aet al.(2001) Summary of workshop findings for porcine T-lymphocyte-specific monoclonal antibodies.Vet Immunol Immunopathol. 80 (1-2): 35-52.2.Castellano, G.et al.(2010) Therapeutic targeting of classical and lectin pathways of complement protects from ischemia-reperfusion-induced renal damage.Am J Pathol. 176: 1648-59.3.Inman, C.F.et al.(2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.J Leukoc Biol. 88 (3): 571-8.4.Kick ARet al.(2011) Evaluation of peripheral lymphocytes after weaning and vaccination forMycoplasma hyopneumoniae.Res Vet Sci. 91 (3): e68-72.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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BIO-RAD MCA1749F Mouse anti Pig CD4 Alpha:FITC,Mouse anti Pig CD4 Alpha:FITC

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