NotI内切酶 Thermo Fisher ER0591,NotI(FauNDⅠ) (10 U/µL)/NotI 内切酶

2024-10-24

NotI(FauNDⅠ) (10 U/µL)/NotI 内切酶

货号:ER0591,ER0592,ER0595

规格:300 units,1500 units,500 units

价格:533,2112,849

产品类型:内切酶

品牌:Thermo Fisher

Thermo Scientific NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in O buffer (Isoschizomers: CciNI). SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as aFastDigest enzymefor rapid DNA digestion.Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.Note:For methylation sensitivity, refer to product specifications.NotI限制性内切酶能够识别GC^GGCCGC位点,在Tango缓冲体系(+DTT)中于37°C达到最佳切割效果(同裂酶:FauNDI)。Thermo Scientific传统限制性内切酶产品包含了大量的高品质内切酶,这些酶经过优化,可在五种缓冲液体系中的其中一种中进行酶切,此外,还随酶提供通用Tango缓冲液,以便进行双酶切。所有内切酶在推荐的缓冲液和反应条件下都具有100%的活性。为保证内切酶的性能稳定,Thermo Scientific限制性内切酶反应缓冲液还预先混入了BSA,以增强酶的稳定性并结合可能存在于DNA制备产物中的污染物。注意事项:使用BseLI消化pTZ19RJL2质粒DNA进行分析。超螺旋质粒相比线性DNA,可能需要5倍以上的NotI酶量才能实现完全消化。
优点:
- Superior quality—stringent quality control and industry leading manufacturing process- Convenient color-coded Five Buffer System- Includes universal Tango buffer for double-digestions- BSA premixed in reaction buffers- Wide selection of restriction endonuclease specificities- 卓越的质量—严格的质量控制和行业领先的生产工艺- 便利的颜色编码的五缓冲液系统- 包括用于双酶切的通用型 Tango 缓冲液- 在反应缓冲液中预混合 BSA- 多种特异性限制性核酸内切酶可供选择
数据:

技术参数

产品优点 1. 优异的质量—严格的质量控制和业内领先的生产流程 2. 使用颜色标记的五大便捷缓冲系统 3. 包含适于双酶切的通用型Tango缓冲液 4. 反应缓冲液中预先混入BSA 5. 种类齐全,特异性限制性内切酶的广泛选择

产品应用 • 分子克隆 • 限制性酶切位点作图(Restriction site mapping) • 基因分型 • Southern blotting • 限制性片段长度多态性(RFLP) • SNP

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