BIO-RAD MCA1653F Mouse anti Bovine CD4:FITC,Mouse anti Bovine CD4:FITC-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

BIO-RAD MCA1653F Mouse anti Bovine CD4:FITC,Mouse anti Bovine CD4:FITC

2024-10-22

Mouse anti Bovine CD4:FITC

货号：MCA1653F

规格：0.1 mg

价格：3740

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD4

物种：Bovine

宿主：小鼠

抗体亚型：IgG2a

克隆号：CC8

荧光染料：FITC

产品介绍
Mouse anti Bovine CD4 antibody, clone CC8recognizes bovine CD4, the homolog of human CD4 and immunoprecipitates a ~50 kDa molecule. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected. Mouse anti Bovine CD4 antibody, clone CC8 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Eskraet al.1991).A mutation in the bovine CD4 gene resulting in an amino acid substitution at A324 T, located in the D4 domain of the CD4 gene product can occur. This mutation results in lowered binding of Mouse anti Bovine CD4 antibody, clone CC8 to CD4 in Japanese Black (JB) cattle where this mutation has been identified (Kato-Mori,et al.. 2020). CD4 in JB cattle can be identified using clone CACT138A (MCA6081) whose binding to bovine CD4 is unaffected by the A324T mutation (Kato-Mori,et al.. 2020).
产品详情
Target Species	Bovine
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Immunogen	Bovine lymphocytes.
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Approx. Protein Concentrations	IgG concentration 0.1 mg/ml
Fusion Partners	Spleen cells from an immunized mouse were fused with cells of the mouse NS1 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√		Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
同型对照
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control:FITC	MCA928F	F	100 Tests/1ml
文献
1.Endsley, J.J.et al.(2004) Characterization of bovine homologues of granulysin and NK-lysin.J Immunol. 173 (4): 2607-14.2.Bensaid, A. & Hadam, M. (1991) Individual antigens of cattle. Bovine CD4 (BoCD4).Vet Immunol Immunopathol. 27 (1-3): 51-4.3.Eskra, L.et al.(1991) Effect of monoclonal antibodies onin vitrofunction of T-cell subsets.Vet Immunol Immunopathol. 27 (1-3): 215-22.4.Howard, C.J.et al.(1991) Summary of workshop findings for leukocyte antigens of cattle.Vet Immunol Immunopathol. 27 (1-3): 21-7.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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BIO-RAD MCA1653F Mouse anti Bovine CD4:FITC,Mouse anti Bovine CD4:FITC

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