BIO-RAD MCA1649F Mouse anti Bovine CD62L:FITC,Mouse anti Bovine CD62L:FITC-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

BIO-RAD MCA1649F Mouse anti Bovine CD62L:FITC,Mouse anti Bovine CD62L:FITC

2024-10-22

Mouse anti Bovine CD62L:FITC

货号：MCA1649F

规格：0.1 mg

价格：3740

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD62L

物种：Bovine

宿主：小鼠

抗体亚型：IgG1

克隆号：CC32

荧光染料：FITC

产品介绍
Mouse anti Bovine CD62L antibody, clone CC32recognizes bovine L-selectin, also known as CD62L, Leukocyte-endothelial cell adhesion molecule 1, LECAM-1 or Lymph node homing receptor.Bovine CD62L is a 370 amino acid ~90 kDa, single pass type I transmembrane glycoprotein bearing a singleC-type lectindomain, anEGF-likedomain and twoSushidomains (UniProt: P98131). Immunoprecipitation of peripheral blood mononuclear cell lysates with Mouse anti Bovine CD62L antibody, clone CC32 reveals a molecule of ~90 kDa when run on polyacrylamide gels under reducing conditions, slightly larger than the murine and human CD62L homologues. Bovine CD62L is expressed on subpopulations of T-lymphocytes expressing CD2, CD4 and CD8. WC1 positive γ/δ T cells also express CD62L as do a subpopulation of WC3+veB-lymphocytes and all peripheral blood monocytes (Howardet al.1992).Mouse anti Bovine CD62L antibody, clone CC32 has also been used successfully for the identification of CD62L on ovine peripheral blood cells by flow cytometry (Hallidayet al.2005).
产品详情
Target Species	Bovine
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Immunogen	Human monocytes
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Approx. Protein Concentrations	IgG concentration 0.1 mg/ml
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the NS1 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat	1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
同型对照
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control:FITC	MCA928F	F	100 Tests/1ml
文献
1.Sopp, P. & Howard, C.J. (2001) IFN gamma and IL-4 production by CD4, CD8 and WC1 gamma delta TCR(+) T cells from cattle lymph nodes and blood.Vet Immunol Immunopathol. 81 (1-2): 85-96.2.Toka, F.N.et al.(2011) Rapid and Transient Activation of {gamma}{delta} T Cells to IFN-{gamma} Production, NK Cell-Like Killing, and Antigen Processing during Acute Virus Infection.J Immunol. 186: 4853-61.3.Vesosky, B.et al.(2003) Activation marker expression on bovine peripheral blood gammadelta T cells during post-natal development and following vaccination with a commercial polyvalent viral vaccine.Dev Comp Immunol. 27: 439-47.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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BIO-RAD MCA1649F Mouse anti Bovine CD62L:FITC,Mouse anti Bovine CD62L:FITC

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