产品介绍 | | |
| Mouse anti Human CD20 antibody, clone 2H7recognizes the human CD20 cell surface antigen, a 33-37 kDa non-glycosylated phosphoprotein.The CD20 antigen is expressed during pre-B-cell development. It is present on both resting and activated B-cells but is lost prior to terminal B-cell differentiation into plasma cells.The epitope recognized by clone 2H7 has been mapped to the following sequence found in the large extracellular loop of human CD20: YNCEPANPSEKNSPST. Furthermore it appears that Mouse anti Human CD20 antibody, clone 2H7 only recognizes human CD20 in its native oligomeric form (Polyaket al.2002). |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Rhesus Monkey | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG conjugated to Pacific Blue® - liquid. |
Preparation | MCA1710PB: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant MCA1710PBT: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
|
Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide 1% Bovine Serum Albumin |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Pacific Blue® | 410 | 455 |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | 1/5 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells .
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| 同型 |
Description | Product Code | Applications | Pack Size |
Mouse IgG2b Negative Control:Pacific Blue® | MCA691PB | F | 100 Tests/1ml |
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| 文献 |
| 1. Greig, B.et al.(2014) Stabilization media increases recovery in paucicellular cerebrospinal fluid specimens submitted for flow cytometry testing.Cytometry B Clin Cytom. 86: 135-8.2. van den Akker, E.et al.(2010) The majority of the in vitro erythroid expansion potential resides in CD34(-) cells, outweighing the contribution of CD34(+) cells and significantly increasing the erythroblast yield from peripheral blood samples.Haematologica. 95: 1594-8.3. Jaramillo, M.C.et al.(2015) Manganese (III) meso-tetrakis N-ethylpyridinium-2-yl porphyrin acts as a pro-oxidant to inhibit electron transport chain proteins, modulate bioenergetics, and enhance the response to chemotherapy in lymphoma cells.Free Radic Biol Med. 83: 89-100.4. Cecchinato, V.et al.(2017) Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization.J Immunol. 198 (1): 184-195.5. Kohler, S.L.et al.(2016) Germinal Center T Follicular Helper Cells Are Highly Permissive to HIV-1 and Alter Their Phenotype during Virus Replication.J Immunol. 196 (6): 2711-22.6. Grobárová Vet al.(2016) Quambalarine B, a Secondary Metabolite fromQuambalaria cyanescenswith Potential Anticancer Properties.J Nat Prod. 79 (9): 2304-14.7. Popov, J.et al.(2017) Unique therapeutic properties and preparation methodology of multivalent rituximab-lipid nanoparticles.Eur J Pharm Biopharm. 117: 256-69.8. Sieg, M.et al.(2019) A New Genotype of Feline Morbillivirus Infects Primary Cells of the Lung, Kidney, Brain and Peripheral Blood.Viruses. 11 (2) Feb 09 [Epub ahead of print]. |
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