F4/80 Monoclonal Antibody (BM8), eFluor 450 Thermo,F4/80 Monoclonal Antibody (BM8), eFluor 450

2024-10-22

F4/80 Monoclonal Antibody (BM8), eFluor 450

货号:48-4801-80,48-4801-82

规格:25μg,100μg

价格:1448,3190

产品类型:流式抗体

品牌:Thermo Fisher

物种:小鼠

宿主:大鼠

抗体亚型:IgG2a, kappa

荧光染料:Alexa Fluor 488

抗体类型 一抗同型对照:Rat IgG2a kappa Isotype Control (eBR2a), eFluor 450
浓度:0.2 mg/mL用法:

0.5 μg/test (Flow);
产品详细信息Description: The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 160 kDa surface receptor. It belongs to the EGF-TM7 family of proteins. As such it contains seven EGF-like domains on its extracellular N-terminus, seven transmembrane spanning sequences, and an intracellular C-terminal domain showing homology to other TM7 superfamily members. The F4/80 antigen is expressed by a majority of mature macrophages and is one of the best markers for this population of cells. However, other cell types, such as peritoneal eosinophils, Langerhans cells, and some other dendritic cell subtypes, have been reported to express this antigen as well. Expression of F4/80 commences during early myeloid development in vivo and can be upregulated on BM cells stimulated in vitro with M-CSF. Some populations of macrophages, especially in the lymphoid microenvironment, may be devoid of F4/80.Applications Reported: This BM8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This BM8 antibody has been tested by flow cytometric analysis of mouse resident peritoneal exudate cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells
数据
F4/80 Antibody (48-4801-82) in FlowStaining of mouse resident peritoneal exudate cells with Anti-Mouse CD11b PE (Product # 12-0112-82) and 0.25 µg of Rat IgG2a K Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or 0.25 µg of Anti-Mouse F4/80 Antigen eFluor® 450 (right). Cells in the large scatter population were used for analysis.
推荐产品:
二抗荧光二抗免疫组化一抗标签抗体
参考文献:
1.Journal of orthopaedic research : official publication of the Orthopaedic Research SocietyHigh fat diet accelerates cartilage repair in DBA/1 mice."Published figure using F4/80 monoclonal antibody (Product # 48-4801-82) in Immunohistochemistry"2.Scientific reportsThe Role of TSLP in IL-13-induced atopic march."Published figure using F4/80 monoclonal antibody (Product # 48-4801-82) in Immunohistochemistry"3.The Journal of clinical investigationCell-specific translational profiling in acute kidney injury."Published figure using F4/80 monoclonal antibody (Product # 48-4801-82) in Immunohistochemistry"4.JCI insightGDF15 is elevated in mice following retinal ganglion cell death and in glaucoma patients."Published figure using F4/80 monoclonal antibody (Product # 48-4801-82) in Immunohistochemistry (Frozen)"5.Nature communicationsTumour-associated macrophages act as a slow-release reservoir of nano-therapeutic Pt(IV) pro-drug."48-4801 was used in Flow cytometry/Cell sorting to reveal that TAMs serve as a slow release reservoir, accumulating significant vehicle from which DNA-damaging payload targets neighbouring tumour cells."AuthorsMiller MA,Zheng YR,Gadde S,Pfirschke C,Zope H,Engblom C,Kohler RH,Iwamoto Y,Yang KS,Askevold B,Kolishetti N,Pittet M,Lippard SJ,Farokhzad OC,Weissleder R

技术参数
© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:https://gibcohyclone.cn/thread-40351.htm
产品询价需求提交
返回
扫码添加客服微信,咨询产品报价