Bio-Rad MCA1683GT BRCA1抗体,Mouse anti Human BRCA1 antibody, clone MS13-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1683GT BRCA1抗体,Mouse anti Human BRCA1 antibody, clone MS13

2024-10-22

Mouse anti Human BRCA1 antibody, clone MS13

货号：MCA1683GT

规格：50 µg

价格：3485

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原： BRCA1

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1

克隆号：MS13

荧光染料：Purified

产品介绍
Mouse anti Human BRCA1 antibody, clone MS13recognizes the human tumor suppressor protein BRCA-1, also known as Breast cancer type 1 susceptibility protein or RING finger protein 53. BRCA1 is a 1863 amino acid, ~220 kDa E3 ubiquitin-protein ligase playing a central role in DNA repair (Tibbettset al.2000), expressed in the nucleus during the S/G2 phase of the cell cycle (Durrant and Nickoloff 2005). Normal BRCA-1 acts as a tumor suppressor protein and mutation or dysregulation of BRCA1 may indicate high risk of development of disease, including breast cancer (Budhram-Mahadeoet al.1999). Mouse anti human BRCA-1, clone MS13 recognizes an epitope within the 304 amino acid N-Terminal (NT) region (Yoshikawaet al.1999) of human BRCA1.BRCA1 is expressed numerous organs including mammary and ovarian tissues (Mikiet al.1994) . Mutations in the BRCA-1 gene are associated with hereditary breast and ovarian cancers. Secondary cancers such as prostate or melanoma also frequently arise in the latter stages of both male and female breast cancer patients (Beneventoet al.2012), but genetic screening and increased awareness of preventative surgery, can reduce this risk significantly (Scheueret al.2002). However, in males pancreatic and prostate cancer appear to be more strongly associated with BRCA2 gene mutations (Gallagheret al.2010).BRCA1 is a key marker of triple-negative breast cancer (TNBC), a high risk aggressive cancer which makes up about 15% of invasive breast cancers, and lacks the benefit of specific targeted therapy (Duffyet al.2012). Triple-negative tumors are predominantly basal-like, poorly differentiated and of higherhistological grade.Mouse anti human BRCA1 antibody, clone MS13 is suitable for use in the immunohistochemical staining of human tissues (Fraseret al.2003) and for immunofluorescence in multiple cell lines (Scullyet al.1996).
产品详情
Target Species	Human
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	TRIS-glycine buffered saline, NaCl
Preservative Stabilisers	<0.1% Sodium Azide (NaN3)
Immunogen	Recombinant protein corresponding to the N-Terminal region of human BRCA1.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunized mice were fused with cells of the mouse NSI myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√
Immunofluorescence	√		1/500
Immunohistology - Frozen¹	√
Immunohistology - Paraffin²	√		1/50
Immunoprecipitation	√
Western Blotting	√		0.5ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Histology Positive Control TissueBreast carcinoma Western BlottingMouse anti Human BRCA1 detects a band of approximately 220kDa in HeLa nuclear extract. Some cell lysates may also show an uncharacterized band of approximately 65kDa
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control	MCA928	Flow	100 Tests
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Human Seroblock	BUF070A	Flow	50 Tests
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文献
1. Scully, R.et al.(1996) Location of BRCA1 in human breast and ovarian cancer cells.Science. 272 (5258): 123-6.2. Yoshikawa, K.et al.(1999) Reduction of BRCA1 protein expression in Japanese sporadic breast carcinomas and its frequent loss in BRCA1-associated cases.Clin Cancer Res. 5 (6): 1249-61.3. Ribeiro-Silva, A.et al.(2005) p63 correlates with both BRCA1 and cytokeratin 5 in invasive breast carcinomas: further evidence for the pathogenesis of the basal phenotype of breast cancer.Histopathology. 47: 458-664. Oliveira-Costa, J.P.et al.(2010) Significance of topoisomerase IIIβ expression in breast ductal carcinomas: strong associations with disease-specific survival and metastasis.Hum Pathol. 41: 1624-30.5. Oliveira-Costa, J.P.et al.(2014) BRCA1 and γH2AX as independent prognostic markers in oral squamous cell carcinoma.Oncoscience. 1 (5): 383-91.6. Ribeiro-Silva, A.et al.(2006) Expression of checkpoint kinase 2 in breast carcinomas: correlation with key regulators of tumor cell proliferation, angiogenesis, and survival.Histol Histopathol. 21 (4): 373-82.7. Meng, Z.H.et al.(2004) Aberrations of breast cancer susceptibility genes occur early in sporadic breast tumors and in acquisition of breast epithelial immortalization.Genes Chromosomes Cancer. 41 (3): 214-22.

技术参数

产品应用 IF;IP;P;WB

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Bio-Rad MCA1683GT BRCA1抗体,Mouse anti Human BRCA1 antibody, clone MS13

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