Bio-Rad MCA1561 CD154 抗体,Mouse anti Human CD154 antibody, clone TRAP-1-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1561 CD154 抗体,Mouse anti Human CD154 antibody, clone TRAP-1

2024-10-22

Mouse anti Human CD154 antibody, clone TRAP-1

货号：MCA1561

规格：0.2 mg

价格：3783

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD154

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号：TRAP-1

荧光染料：Purified

产品介绍
Mouse anti Human CD154 antibody, clone TRAP-1recognises the human CD40 ligand, also known as CD154, TNF-related activation protein (TRAP) or T-cell antigen Gp39. CD154 is a 261 amino acid ~32 kDa single pass, type-1 transmembrane glycoprotein (UniProt: P29965). CD154 is expressed on activated T lymphocytes, predominantly CD4 +ve and also on some basophils and mast cells.Mouse anti Human CD154 antibody, clone TRAP-1 binds to CD154 at an epitope distinct from the CD40 binding site (Kroczeket al.1994).
产品详情
Target Species	Human
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from ascites
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	<0.1% Sodium Azide (NaN3)0.1% Bovine Serum Albumin
Immunogen	Mouse myeloma cell line transfected with human CD40L (CD154)
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3X63.Ag 8653 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	1/10	1/50
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control	MCA928	Flow	100 tests
文献
1.Hermann, P.et al.(1993) Expression of a 32-kDa ligand for the CD40 antigen on activated human T lymphocytes.Eur J Immunol. 23 (4): 961-4.2.Lane, P.et al.(1992) Activated human T cells express a ligand for the human B cell-associated antigen CD40 which participates in T cell-dependent activation of B lymphocytes.Eur J Immunol. 22 (10): 2573-8.3. Kroczek, R.Aet al.(1994) Defective expression of CD40 ligand on T cells causes "X-linked immunodeficiency with hyper-IgM (HIGM1)".Immunol Rev. 138: 39-59.4. Houtkamp, M.A.et al.(2001) Interleukin-15 expression in atherosclerotic plaques: an alternative pathway for T-cell activation in atherosclerosis?Arterioscler Thromb Vasc Biol. 21: 1208-13.5. Yarwood, H.et al.(2000) Resting and activated T cells induce expression of E-selectin and VCAM-1 by vascular endothelial cells through a contact-dependent but CD40 ligand-independent mechanism.J Leukoc Biol. 68: 233-42.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Bio-Rad MCA1561 CD154 抗体,Mouse anti Human CD154 antibody, clone TRAP-1

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