Bio-Rad MCA1658 IL-1 Beta抗体,Mouse anti Sheep Interleukin-1 beta antibody, clone 1D4-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1658 IL-1 Beta抗体,Mouse anti Sheep Interleukin-1 beta antibody, clone 1D4

2024-10-22

Mouse anti Sheep Interleukin-1 beta antibody, clone 1D4

货号：MCA1658

规格：0.25 mg

价格：5670

产品类型：流式抗体

品牌：Bio-Rad

抗原：IL-1 Beta

物种：绵羊

宿主：小鼠

抗体亚型：IgG1

克隆号：1D4

荧光染料：Purified

产品介绍
Mouse anti Sheep Interleukin-1 beta antibody, clone 1D4recognizes ovine interleukin-1 beta, and shows no cross-reactivity with ovine IL-6, IL-8, TNF alpha or MCP-1.Mouse anti Sheep Interleukin-1 beta antibody, clone 1D4 demonstrates partial neutralizing activity of ovine IL-1 beta.
产品详情
Target Species	Sheep
Species Cross-Reactivity	Target Species	Cross Reactivity
Goat	√
Bovine	√
Horse	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers	<0.1% Sodium Azide (NaN3)
BufferSolution	Phosphate buffered saline
Immunogen	Recombinant ovine IL-1 Beta
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry 1	√		1/10
ELISA	√
Immunohistology - Frozen	√
Western Blotting	X
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.¹Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product CodeBUF09) for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100ul. ELISAMouse anti Interleukin-1 beta antibody, clone 1D4 may be used as a capture antibody in a bovine IL-1 beta sandwich ELISA together with Rabbit anti Bovine interleukin-1β antibody (AHP851B) as the detection reagent for evaluation of IL-1β levels in bovine samples together with recombinant Bovine interleukin-1β (PBP008) used as standards.Alternatively, Mouse anti Interleukin-1 beta antibody, clone 1D4 can be used as a capture reagent together with Rabbit anti Ovine interleukin-1β antibody (AHP423) as a detection reagent for the evaluation of IL-1β levels in ovine, bovine or caprine samples, again utilizing recombinant bovine IL-1&beta (PBP008) as an internal standard.
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control	MCA928	Flow	100 Tests
文献
1. Doull, L.et al.(2015) Late production of CXCL8 in ruminant oro-nasal turbinate cells in response toChlamydia abortusinfection.Vet Immunol Immunopathol. 168 (1-2): 97-102.2. Xu, A.et al.(2015) The Ovine Fetal and Placental Inflammatory Response to Umbilical Cord Occlusions With Worsening Acidosis.Reprod Sci. 22 (11): 1409-20.3. Sobotta, K.et al.(2016)Coxiella burnetii.Infects Primary Bovine Macrophages and Limits Their Host Cell Response.Infect Immun. 84 (6): 1722-34.4. Cortes, M.et al.(2017) RNAseq profiling of primary microglia and astrocyte cultures in near-term ovine fetus: A glialin vivo-in vitromulti-hit paradigm in large mammalian brain.J Neurosci Methods. 276: 23-32.5. Canal AMet al.(2017) Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with naturalMycobacterium bovisinfection.Res Vet Sci. 110: 34-39.6. Cao, M.et al.(2019) α7 Nicotinic Acetylcholine Receptor Signaling Modulates Ovine Fetal Brain Astrocytes Transcriptome in Response to Endotoxin.Front Immunol. 10: 1063.7. Stassi, A.F.et al.(2019) Follicular structures of cows with cystic ovarian disease present altered expression of cytokines.Zygote. 15: 1-14.8. Ciliberti, M.G.et al.(2022) Green extraction of bioactive compounds from wine lees and their bio-responses on immune modulation usingin vitro.sheep model.J Dairy Sci. Mar 17 [Epub ahead of print].9. Damiano, S.et al.(2022) Red orange and lemon extract preserve from oxidative stress, DNA damage and inflammatory status in lambsIt J Anim Sci. 21 (1): 934-42.

技术参数

产品应用 ELISA;IHC;Flow;WB

产品类型 Monoclonal Antibody

推荐用于 ELISA;IHC;Flow;WB

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Bio-Rad MCA1658 IL-1 Beta抗体,Mouse anti Sheep Interleukin-1 beta antibody, clone 1D4

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