Bio-Rad MCA1558P PCNA抗体,mouse anti PCNA:HRP

2024-10-22

mouse anti PCNA:HRP

货号:MCA1558P

规格:0.1 mg

价格:3247

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原: PCNA

物种:人/小鼠

宿主:小鼠

抗体亚型:IgG2a

克隆号:PC10

荧光染料:HRP

产品介绍
Mouse anti PCNA antibody, clone PC10recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kDa nuclear protein vital for cellular DNA synthesis at the replication fork (Liet al.1995) through its interaction withFEN1(Wuet al.1996). PCNA is the auxilliary protein for DNA polymerase δ (Bravoet al.1987).PCNA is highly conserved between mammalian species and other vertebrates. Mouse anti PCNA antibody, clone PC10 has been used for the detection of PCNA in a number of species including human, rat, mouse (Parket al.2008), chicken (Franz-Odendaal 2008) and abalone (Harriset al.2006).
产品详情

Target Species

Rat

Species Cross-ReactivityTarget SpeciesCross Reactivity
VertebratesExpected from Sequence
InvertebratesExpected from Sequence
Ferret
Chicken
Rabbit

Xenopus

Mouse
Horse
Sheep
Dog
Cat
Cynomolgus monkey
Rhesus Monkey
Hamster
Atlantic Salmon

Human

Bearded Dragon
Corn Snake
Nile Crocodile
N.B. Antibody reactivity and working conditions may vary between species.
Product Form

Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.01% Thiomersal

HRP Stabiliser (BUF052A)

ImmunogenRat PCNA made in the protein A expression vector pR1T2T
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
WB1/1000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
文献

1. Fenton, M.et al.(2001) Cellular senescence after single and repeated balloon catheter denudations of rabbit carotid arteries.Arterioscler Thromb Vasc Biol. 21: 220-6.2. Park, J.H.et al.(2008) Gastric lesions and immune responses caused by long-term infection with Helicobacter heilmannii in C57BL/6 mice.J Comp Pathol. 139: 208-17.3. Izhak, L.et al.(2012) Dissecting the autocrine and paracrine roles of the CCR2-CCL2 axis in tumor survival and angiogenesis.PLoS One. 7: e28305.4. Di-poï, N. & Milinkovitch, M.C. (2016) The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes.Sci Adv. 2 (6): e1600708.5. Guzera, M.et al.(2016)In VitroInfluence of Mycophenolic Acid on Selected Parameters of Stimulated Peripheral Canine Lymphocytes.PLoS One. 11 (5): e0154429.6. Khlebnikov, V. V.et al.(2015) Developmental Characteristics of the Hypothalamo-Hypophyseal-Adrenal System in Chronic Heterotypical StressNeuroscience and Behavioral Physiology. 46 (1): 100-5.7. Nakatsuka, M. & Kumabe, S (2018) Histological Alterations from Condyle Repositioning with Functional Appliances in Rats.J Clin Pediatr Dent. 42 (5): 391-7.8. Falbo, L.et al.(2020) SSRP1-mediated histone H1 eviction promotes replication origin assembly and accelerated development.Nat Commun. 11 (1): 1345.

技术参数

产品应用 WB

产品类型 Monoclonal Antibody

推荐用于 WB

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