| 产品介绍 | | |
| Mouse anti Human CD105 antibody, clone SN6recognizes human endoglin, also known as CD105. CD105 is a glycoprotein homodimer of ~95 kDa subunits expressed by endothelial cells, activated monocytes and some leukemia cells. |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Horse | √ |
| Cynomolgus monkey | √ |
| Rhesus Monkey | √ |
| Primate | Expected from Sequence |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG conjugated to RPE-Alexa Fluor 647 - lyophilized |
| Reconstitution | Reconstitute with 1.0 ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin5% Sucrose |
| Immunogen | Partially purified cell membrane antigens from fresh leukemia cells |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE-Alexa Fluor®647 488nm laser | 496 | 667 |
| RPE-Alexa Fluor®647 561nm laser | 546 | 667 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul
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| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:RPE-Alexa Fluor® 647 | MCA928P647 | Flow | 100 Tests/1ml |
| 相关产品 |
| Description | Product Code | Applications | Pack Size |
| Human Seroblock | BUF070A | Flow | 50 Tests |
| Human Seroblock | BUF070B | Flow | 200 Tests |
| 数据 |
| Figure A. FITC conjugated Mouse anti Human CD31 (MCA1738F) and PE-Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928P647). Figure B. FITC conjugated Mouse anti Human CD31 (MCA1738F) and PE-Alexa Fluor 647 conjugated Mouse anti Human CD105 (MCA1557P647). All experiments performed on HUVECs gated on live single cells, in the presence of 10% human serum. Data acquired on theZE5 Cell Analyzer. |
| 文献 |
| 1.Pierelli, L.et al.(2000) Modulation of bcl-2 and p27 in human primitive proliferating hematopoietic progenitors by autocrine TGF-beta1 is a cell cycle-independent effect and influences their hematopoietic potential.Blood 95: 3001-9.2.May, J.E.et al.(2018) Chemotherapy-induced genotoxic damage to bone marrow cells: long-term implications.Mutagenesis. 33 (3): 241-251.3.Hauser, P.V.et al.(2010) Stem cells derived from human amniotic fluid contribute to acute kidney injury recovery.Am J Pathol. 177: 2011-21.4.Jin, H.J.et al.(2010) GD2 expression is closely associated with neuronal differentiation of human umbilical cord blood-derived mesenchymal stem cells.Cell Mol Life Sci. 67 (11): 1845-58.5.Nagano, M.et al.(2007) Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood.Blood 110 (1): 151-60.6.Braun, J.et al.(2010) Evaluation of the osteogenic and chondrogenic differentiation capacities of equine adipose tissue-derived mesenchymal stem cells.Am J Vet Res. 71 (10): 1228-36.7.Diaz-Romero, J.et al.(2008) Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells.J Cell Physiol. 214: 75-83.8.Agha-Hosseini, F.et al.(2010)In vitroisolation of stem cells derived from human dental pulp.Clin Transplant. 24: E23-8.9.Arufe, M.C.et al.(2010) Chondrogenic potential of subpopulations of cells expressing mesenchymal stem cell markers derived from human synovial membranes.J Cell Biochem. 111: 834-45.10.Balmayor, E.R.et al.(2011) Synthesis and functionalization of superparamagnetic poly-ε-caprolactone microparticles for the selective isolation of subpopulations of human adipose-derived stem cells.J R Soc Interface 8: 896-908. |
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