Bio-Rad MCA1553PE IL-2抗体,Rat anti human IL-2:PE

2024-10-22

Rat anti human IL-2:PE

货号:MCA1553PE

规格:100 Tests/1ml

价格:3188

产品类型:流式抗体

品牌:Bio-Rad

抗原: IL-2

物种:人

宿主:大鼠

抗体亚型:IgG2a

克隆号:MQ1-17H12

荧光染料:PE

产品介绍
Rat anti Human Interleukin-2 antibody, clone MQ1-17H12recognizes human interleukin-2 (IL-2), also known as T-cell growth factor or Aldesleukin. IL-2 is a 153 amino acid secreted cytokine produced by T-cells following antigenic or mitogenic stimulation. It plays a crucial role in T-cell proliferation and immune regulation.Rat anti Human Interleukin-2 antibody, clone MQ1-17H12 has been sucessfully used for the detection and measurement of native and recombinant human IL-2 using flow cytometry and ELISA where it ascts as the capture reagent in a very sensitive assay for IL-2 determination in human samples. IL-2 is used extensively for the treatment of patients with metastatic melanoma (Atkinset al.1999) and renal cancer with reproducible benificial effects (Rosenberg 2014). Monitoring of IL-2 levels in such patients is required for optimization of treatment regimens (Lotzeet al.1986). Rat anti Human Interleukin-2 antibody, clone MQ1-17H12 is also useful for evaluating levels of IL-2 in patients with autoimmune conditions including lupus erythematosus and rheumatoid arthritis where there may be an impaired ability to produce IL-2 (Talalet al.1983).
产品详情

Target Species

Human

Species Cross-ReactivityTarget SpeciesCross Reactivity
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

ReconstitutionReconstitute with 1.0ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN3)

1% Bovine Serum Albumin

5% Sucrose

Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
RPE 488nm laser496578

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
Prior to reconstitution store at +4oC.After reconstitution store at +4oC.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry1Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm™(Product CodeBUF09) for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100ul.
同型
DescriptionProduct CodeApplicationsPack Size
Rat IgG2a Negative Control:RPE

MCA6005PE

Flow100 Tests
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数据

Figure A. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 5 hours were stained with Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and PE conjugated Rat IgG2a (MCA6005PE). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A)for 5 hours were stained with Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and PE conjugated Mouse anti Human IL-2 (MCA1553PE). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer
文献

1. Garbe, Y.et al.(2011) Semiallogenic fusions of MSI(+) tumor cells and activated B cells induce MSI-specific T cell responses.BMC Cancer. 11: 410.2. Moncrieffe, H.et al.(2010) High expression of the ectonucleotidase CD39 on T cells from the inflamed site identifies two distinct populations, one regulatory and one memory T cell population.J Immunol. 185 (1): 134-43.

    技术参数

    产品应用 Flow Cytometry

    产品类型 Monoclonal Antibody

    推荐用于 Flow Cytometry

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