Mouse IgG2b kappa Isotype Control (eBMG2b), PE

货号：12-4732-81,12-4732-82

规格：50μg,100μg

价格：925,1545

产品类型：小鼠同型对照

品牌：Thermo Fisher

物种：其它

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：eBMG2b

荧光染料：PE

类型:		同型对照：
浓度：	0.2 mg/mL	用法：	Assay-Dependent (Ctrl)；Assay-Dependent (Flow)

产品详细信息Description: The monoclonal mouse IgG2b, kappa is useful as an isotype control immunoglobulin.Applications Reported: This mouse IgG2b isotype control has been reported for use in flow cytometric analysis.Applications Tested: This Mouse IgG2b K Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells and can be used at the same concentration as the experimental antibody.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser,Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

数据

Mouse IgG2b kappa Isotype Control (12-4732-82) in FlowStaining of a mixture of normal human iPSC and the murine C2C12 cell line with Anti-Human TRA-1-60 (Podocalyxin) PerCP-eFluor® 710 (Product # 46-8863-82) and Mouse IgG2b kappa Isotype Control, PE (Product # 12-4732-82) (left) or Anti-Human PCDH1 (Protocadherin 1) PE (right). Viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.

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参考文献：

1.Scientific reportsPlatelets confound the measurement of extracellular miRNA in archived plasma."12-4732 was used in Flow cytometry/Cell sorting to show that a single freeze/thaw cycle of plasma dramatically increases the number of platelet-derived MPs, contaminates the extracellular miRNA pool, and profoundly affects the levels of miRNAs detected."AuthorsMitchell AJ,Gray WD,Hayek SS,Ko YA,Thomas S,Rooney K,Awad M,Roback JD,Quyyumi A,Searles CD2.Experimental and therapeutic medicineEffect of cold stress on immunity in rats."12-4732 was used in Flow cytometry/Cell sorting to determine the effect of a sudden ambient temperature drop on the levels of stress hormones and T-lymphocyte cytokines in the plasma of mice."AuthorsHu GZ,Yang SJ,Hu WX,Wen Z,He D,Zeng LF,Xiang Q,Wu XM,Zhou WY,Zhu QX3.Nucleic acids researchMulti-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells."12-4732 was used in Flow cytometry/Cell sorting to assess the efficiency of homologous recombination after trialling different targeting vector design parameters."AuthorsByrne SM,Ortiz L,Mali P,Aach J,Church GM4.Veterinary researchImmunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells."12-4732 was used in Flow cytometry/Cell sorting to demonstrate that L. casei modulates the interaction between IECs and immune cells during the immune response."AuthorsHosoya S,Villena J,Shimazu T,Tohno M,Fujie H,Chiba E,Shimosato T,Aso H,Suda Y,Kawai Y,Saito T,Alvarez S,Ikegami S,Itoh H,Kitazawa H5.BloodPML4 facilitates erythroid differentiation by enhancing the transcriptional activity of GATA-1."12-4732 was used as a Control in experiments to determine the role promyelocytic leukemia protein (PML) plays in erythropoiesis- specifically PML4, a specific isoform expressed in erythroid cells."AuthorsWu J,Zhou LQ,Yu W,Zhao ZG,Xie XM,Wang WT,Xiong J,Li M,Xue Z,Wang X,Zhang P,Mao BB,Hao DL,Lv X,Liu DP

技术参数

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